Incubation of Ovine Scrapie with Environmental Matrix Results in Biological and Biochemical Changes of PrP(Sc) over Time

Overview

Journal Vet Res

Publisher Biomed Central

Specialty Veterinary Medicine

Date 2015 May 1

PMID 25928902

Citations 4

Authors

Ben C Maddison

John Spiropoulos

Christopher M Vickery

Richard Lockey

Jonathan P Owen

Keith Bishop

Claire A Baker

Kevin C Gough

Affiliations

Soon will be listed here.

Abstract

Ovine scrapie can be transmitted via environmental reservoirs. A pool of ovine scrapie isolates were incubated on soil for one day or thirteen months and eluted prion was used to challenge tg338 mice transgenic for ovine PrP. After one-day incubation on soil, two PrP(Sc) phenotypes were present: G338 or Apl338ii. Thirteen months later some divergent PrP(Sc) phenotypes were seen: a mixture of Apl338ii with either G338 or P338, and a completely novel PrP(Sc) deposition, designated Cag338. The data show that prolonged ageing of scrapie prions within an environmental matrix may result in changes in the dominant PrP(Sc) biological/biochemical properties.

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