Female Reproductive Hormones and Biomarkers of Oxidative Stress in Genital Chlamydia Infection in Tubal Factor Infertility

Overview

Journal J Reprod Infertil

Specialty Reproductive Medicine

Date 2015 May 1

PMID 25927024

Citations 6

Authors

Augusta Chinyere Nsonwu-Anyanwu

Mabel Ayebantoyo Charles-Davies

Victor Olusegun Taiwo

Bin Li

Anthony Alabar Oni

Folashade Adenike Bello

Affiliations

Soon will be listed here.

Abstract

Background: Genital Chlamydia infection (GCI) and the associated pathologies have been implicated in tubal infertility. Though the actual pathologic mechanisms are still uncertain, oxidative stress and other factors have been implicated. The purpose of the study was to determine the possible contribution of female reproductive hormones and biomarkers of oxidative stress in genital Chlamydial infection to tubal occlusion.

Methods: This prospective case control study was carried out by recruiting 150 age matched women grouped into infertile Chlamydia positive women (n = 50), fertile Chlamydia positive women (n = 50) and fertile Chlamydia negative women as controls (n = 50). High vaginal swabs and endocervical swabs were collected for screening Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis, Treponema pallidum, Staphylococcus aureus, and Candida albicans. Sera were collected for estimation of Chlamydia trachomatis antibody, female reproductive hormones [Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH), Oestradiol (E2), Progesterone (P4), Prolactin (PRL)] and biomarkers of oxidative stress [Total Antioxidant Capacity (TAC) and 8-hydroxyl-2-deoxyguanosine (8-OHdG)] by enzyme immunoassay (EIA). Data were analyzed using chi square, analysis of variance and LSD Post hoc to determine mean differences at p = 0.05.

Results: Among women with GCI, higher levels of LH and 8-OHdG were observed in infertile Chlamydia positive women compared to fertile Chlamydia positive women (p < 0.05). Higher levels of LH and 8-OHdG and lower TAC levels were observed in infertile Chlamydia positive women compared to fertile Chlamydia negative controls (p < 0.05).

Conclusion: Mechanisms including oxidative DNA damage and reduced antioxidant capacity may be involved in the pathology of Chlamydia induced tubal damage.

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References

Miller N . Total antioxidant status in plasma and body fluids. Methods Enzymol. 1994; 234:279-93. DOI: 10.1016/0076-6879(94)34095-1. View

Ray S, Lam T, Rotollo J, Phadke S, Patel C, Dontabhaktuni A . Oxidative stress is the master operator of drug and chemically-induced programmed and unprogrammed cell death: Implications of natural antioxidants in vivo. Biofactors. 2005; 21(1-4):223-32. DOI: 10.1002/biof.552210144. View

Van Langendonckt A, Casanas-Roux F, Donnez J . Oxidative stress and peritoneal endometriosis. Fertil Steril. 2002; 77(5):861-70. DOI: 10.1016/s0015-0282(02)02959-x. View

Samra Z, Soffer Y . IgA antichlamydia antibodies as a diagnostic tool for monitoring of active chlamydial infection. Eur J Epidemiol. 1992; 8(6):882-4. DOI: 10.1007/BF00145338. View

Agrawal T, Gupta R, Dutta R, Srivastava P, Bhengraj A, Salhan S . Protective or pathogenic immune response to genital chlamydial infection in women--a possible role of cytokine secretion profile of cervical mucosal cells. Clin Immunol. 2008; 130(3):347-54. DOI: 10.1016/j.clim.2008.10.004. View

Ness R, Smith K, Chang C, Schisterman E, Bass D . Prediction of pelvic inflammatory disease among young, single, sexually active women. Sex Transm Dis. 2006; 33(3):137-42. DOI: 10.1097/01.olq.0000187205.67390.d1. View

Kawaguchi S, Sakumoto R, Okuda K . Induction of the expressions of antioxidant enzymes by luteinizing hormone in the bovine corpus luteum. J Reprod Dev. 2013; 59(3):219-24. PMC: 3934140. DOI: 10.1262/jrd.2012-186. View

Agrawal T, Vats V, Salhan S, Mittal A . The mucosal immune response to Chlamydia trachomatis infection of the reproductive tract in women. J Reprod Immunol. 2009; 83(1-2):173-8. DOI: 10.1016/j.jri.2009.07.013. View

Agarwal A, Gupta S, Sharma R . Role of oxidative stress in female reproduction. Reprod Biol Endocrinol. 2005; 3:28. PMC: 1215514. DOI: 10.1186/1477-7827-3-28. View

10.

Paszkowski T, Traub A, Robinson S, McMaster D . Selenium dependent glutathione peroxidase activity in human follicular fluid. Clin Chim Acta. 1995; 236(2):173-80. DOI: 10.1016/0009-8981(95)98130-9. View

11.

Aten R, Duarte K, Behrman H . Regulation of ovarian antioxidant vitamins, reduced glutathione, and lipid peroxidation by luteinizing hormone and prostaglandin F2 alpha. Biol Reprod. 1992; 46(3):401-7. DOI: 10.1095/biolreprod46.3.401. View

12.

Nsonwu-Anyanwu A, Charles-Davies M, Oni A, Taiwo V, Bello F . Chlamydial infection, plasma peroxidation and obesity in tubal infertility. Ann Ib Postgrad Med. 2014; 9(2):83-8. PMC: 4111027. View

13.

Cooke M, Evans M, Dizdaroglu M, Lunec J . Oxidative DNA damage: mechanisms, mutation, and disease. FASEB J. 2003; 17(10):1195-214. DOI: 10.1096/fj.02-0752rev. View

14.

Straub R . The complex role of estrogens in inflammation. Endocr Rev. 2007; 28(5):521-74. DOI: 10.1210/er.2007-0001. View

15.

Polak G, Koziol-Montewka M, Gogacz M, Blaszkowska I, Kotarski J . Total antioxidant status of peritoneal fluid in infertile women. Eur J Obstet Gynecol Reprod Biol. 2001; 94(2):261-3. DOI: 10.1016/s0301-2115(00)00352-3. View

16.

den Hartog J, Morre S, Land J . Chlamydia trachomatis-associated tubal factor subfertility: Immunogenetic aspects and serological screening. Hum Reprod Update. 2006; 12(6):719-30. DOI: 10.1093/humupd/dml030. View

17.

Beatty W, Byrne G, Morrison R . Repeated and persistent infection with Chlamydia and the development of chronic inflammation and disease. Trends Microbiol. 1994; 2(3):94-8. DOI: 10.1016/0966-842x(94)90542-8. View

18.

Rabiu K, Adewunmi A, Akinlusi F, Akinola O . Female reproductive tract infections: understandings and care seeking behaviour among women of reproductive age in Lagos, Nigeria. BMC Womens Health. 2010; 10:8. PMC: 2851660. DOI: 10.1186/1472-6874-10-8. View

19.

Wira C, Fahey J, Wallace P, Yeaman G . Effect of the menstrual cycle on immunological parameters in the human female reproductive tract. J Acquir Immune Defic Syndr. 2005; 38 Suppl 1:S34-6. DOI: 10.1097/01.qai.0000167040.58181.d5. View

20.

Omoregie R, Egbe C, Igbarumah I, Ogefere H, Okorie E . Prevalence and etiologic agents of female reproductive tract infection among in-patients and out-patients of a tertiary hospital in Benin city, Nigeria. N Am J Med Sci. 2012; 2(10):473-7. PMC: 3339110. DOI: 10.4297/najms.2010.2473. View