Disruption of the Rice Nitrate Transporter OsNPF2.2 Hinders Root-to-shoot Nitrate Transport and Vascular Development

Overview

Journal Sci Rep

Specialty Science

Date 2015 Apr 30

PMID 25923512

Citations 35

Authors

Yuge Li

Jie OuYang

Ya-Yun Wang

Rui Hu

Kuaifei Xia

Jun Duan

Yaqin Wang

Yi-Fang Tsay

Mingyong Zhang

Affiliations

Soon will be listed here.

Abstract

Plants have evolved to express some members of the nitrate transporter 1/peptide transporter family (NPF) to uptake and transport nitrate. However, little is known of the physiological and functional roles of this family in rice (Oryza sativa L.). Here, we characterized the vascular specific transporter OsNPF2.2. Functional analysis using cDNA-injected Xenopus laevis oocytes revealed that OsNPF2.2 is a low-affinity, pH-dependent nitrate transporter. Use of a green fluorescent protein tagged OsNPF2.2 showed that the transporter is located in the plasma membrane in the rice protoplast. Expression analysis showed that OsNPF2.2 is nitrate inducible and is mainly expressed in parenchyma cells around the xylem. Disruption of OsNPF2.2 increased nitrate concentration in the shoot xylem exudate when nitrate was supplied after a deprivation period; this result suggests that OsNPF2.2 may participate in unloading nitrate from the xylem. Under steady-state nitrate supply, the osnpf2.2 mutants maintained high levels of nitrate in the roots and low shoot:root nitrate ratios; this observation suggests that OsNPF2.2 is involved in root-to-shoot nitrate transport. Mutation of OsNPF2.2 also caused abnormal vasculature and retarded plant growth and development. Our findings demonstrate that OsNPF2.2 can unload nitrate from the xylem to affect the root-to-shoot nitrate transport and plant development.

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References

Chiang C, Stacey G, Tsay Y . Mechanisms and functional properties of two peptide transporters, AtPTR2 and fPTR2. J Biol Chem. 2004; 279(29):30150-7. DOI: 10.1074/jbc.M405192200. View

Cai C, Wang J, Zhu Y, Shen Q, Li B, Tong Y . Gene structure and expression of the high-affinity nitrate transport system in rice roots. J Integr Plant Biol. 2008; 50(4):443-51. DOI: 10.1111/j.1744-7909.2008.00642.x. View

Jeong D, An S, Kang H, Moon S, Han J, Park S . T-DNA insertional mutagenesis for activation tagging in rice. Plant Physiol. 2002; 130(4):1636-44. PMC: 166679. DOI: 10.1104/pp.014357. View

Schmollinger S, Muhlhaus T, Boyle N, Blaby I, Casero D, Mettler T . Nitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism. Plant Cell. 2014; 26(4):1410-1435. PMC: 4036562. DOI: 10.1105/tpc.113.122523. View

Wang R, Xing X, Wang Y, Tran A, Crawford N . A genetic screen for nitrate regulatory mutants captures the nitrate transporter gene NRT1.1. Plant Physiol. 2009; 151(1):472-8. PMC: 2735993. DOI: 10.1104/pp.109.140434. View

Chiu C, Lin C, Hsia A, Su R, Lin H, Tsay Y . Mutation of a nitrate transporter, AtNRT1:4, results in a reduced petiole nitrate content and altered leaf development. Plant Cell Physiol. 2004; 45(9):1139-48. DOI: 10.1093/pcp/pch143. View

Xia X, Fan X, Wei J, Feng H, Qu H, Xie D . Rice nitrate transporter OsNPF2.4 functions in low-affinity acquisition and long-distance transport. J Exp Bot. 2014; 66(1):317-31. DOI: 10.1093/jxb/eru425. View

Li S, Qian Q, Fu Z, Zeng D, Meng X, Kyozuka J . Short panicle1 encodes a putative PTR family transporter and determines rice panicle size. Plant J. 2009; 58(4):592-605. DOI: 10.1111/j.1365-313X.2009.03799.x. View

Dechorgnat J, Nguyen C, Armengaud P, Jossier M, Diatloff E, Filleur S . From the soil to the seeds: the long journey of nitrate in plants. J Exp Bot. 2011; 62(4):1349-59. DOI: 10.1093/jxb/erq409. View

10.

Morere-Le Paven M, Viau L, Hamon A, Vandecasteele C, Pellizzaro A, Bourdin C . Characterization of a dual-affinity nitrate transporter MtNRT1.3 in the model legume Medicago truncatula. J Exp Bot. 2011; 62(15):5595-605. DOI: 10.1093/jxb/err243. View

11.

Stacey G, Koh S, Granger C, Becker J . Peptide transport in plants. Trends Plant Sci. 2002; 7(6):257-63. DOI: 10.1016/s1360-1385(02)02249-5. View

12.

Nelson B, Cai X, Nebenfuhr A . A multicolored set of in vivo organelle markers for co-localization studies in Arabidopsis and other plants. Plant J. 2007; 51(6):1126-36. DOI: 10.1111/j.1365-313X.2007.03212.x. View

13.

Alboresi A, Gestin C, Leydecker M, Bedu M, Meyer C, Truong H . Nitrate, a signal relieving seed dormancy in Arabidopsis. Plant Cell Environ. 2005; 28(4):500-12. DOI: 10.1111/j.1365-3040.2005.01292.x. View

14.

Wang Y, Tsay Y . Arabidopsis nitrate transporter NRT1.9 is important in phloem nitrate transport. Plant Cell. 2011; 23(5):1945-57. PMC: 3123939. DOI: 10.1105/tpc.111.083618. View

15.

Krouk G, Lacombe B, Bielach A, Perrine-Walker F, Malinska K, Mounier E . Nitrate-regulated auxin transport by NRT1.1 defines a mechanism for nutrient sensing in plants. Dev Cell. 2010; 18(6):927-37. DOI: 10.1016/j.devcel.2010.05.008. View

16.

Sasaki T, Mori I, Furuichi T, Munemasa S, Toyooka K, Matsuoka K . Closing plant stomata requires a homolog of an aluminum-activated malate transporter. Plant Cell Physiol. 2010; 51(3):354-65. PMC: 2835873. DOI: 10.1093/pcp/pcq016. View

17.

Fang Z, Xia K, Yang X, Suter Grotemeyer M, Meier S, Rentsch D . Altered expression of the PTR/NRT1 homologue OsPTR9 affects nitrogen utilization efficiency, growth and grain yield in rice. Plant Biotechnol J. 2012; 11(4):446-58. DOI: 10.1111/pbi.12031. View

18.

Li J, Fu Y, Pike S, Bao J, Tian W, Zhang Y . The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal from the xylem sap and mediates cadmium tolerance. Plant Cell. 2010; 22(5):1633-46. PMC: 2899866. DOI: 10.1105/tpc.110.075242. View

19.

Almagro A, Lin S, Tsay Y . Characterization of the Arabidopsis nitrate transporter NRT1.6 reveals a role of nitrate in early embryo development. Plant Cell. 2008; 20(12):3289-99. PMC: 2630450. DOI: 10.1105/tpc.107.056788. View

20.

Eckardt N . Nitrogen-Sparing Mechanisms in Chlamydomonas: Reduce, Reuse, Recycle, and Reallocate. Plant Cell. 2014; 26(4):1379. PMC: 4036557. DOI: 10.1105/tpc.114.126334. View