» Articles » PMID: 25918849

Characterization of Tissue-engineered Posterior Corneas Using Second- and Third-harmonic Generation Microscopy

Overview
Journal PLoS One
Date 2015 Apr 29
PMID 25918849
Citations 4
Authors
Affiliations
Soon will be listed here.
Abstract

Three-dimensional tissues, such as the cornea, are now being engineered as substitutes for the rehabilitation of vision in patients with blinding corneal diseases. Engineering of tissues for translational purposes requires a non-invasive monitoring to control the quality of the resulting biomaterial. Unfortunately, most current methods still imply invasive steps, such as fixation and staining, to clearly observe the tissue-engineered cornea, a transparent tissue with weak natural contrast. Second- and third-harmonic generation imaging are well known to provide high-contrast, high spatial resolution images of such tissues, by taking advantage of the endogenous contrast agents of the tissue itself. In this article, we imaged tissue-engineered corneal substitutes using both harmonic microscopy and classic histopathology techniques. We demonstrate that second- and third-harmonic imaging can non-invasively provide important information regarding the quality and the integrity of these partial-thickness posterior corneal substitutes (observation of collagen network, fibroblasts and endothelial cells). These two nonlinear imaging modalities offer the new opportunity of monitoring the engineered corneas during the entire process of production.

Citing Articles

Biomaterials used for tissue engineering of barrier-forming cell monolayers in the eye.

Sasseville S, Karami S, Tchatchouang A, Charpentier P, Anney P, Gobert D Front Bioeng Biotechnol. 2023; 11:1269385.

PMID: 37840667 PMC: 10569698. DOI: 10.3389/fbioe.2023.1269385.


Isolation efficiency of collagenase and EDTA for the culture of corneal endothelial cells.

Santerre K, Proulx S Mol Vis. 2022; 28:331-339.

PMID: 36338664 PMC: 9603909.


Scleral structure and biomechanics.

Boote C, Sigal I, Grytz R, Hua Y, Nguyen T, Girard M Prog Retin Eye Res. 2019; 74:100773.

PMID: 31412277 PMC: 7187923. DOI: 10.1016/j.preteyeres.2019.100773.


Label-free multi-photon imaging of dysplasia in Barrett's esophagus.

Mehravar S, Banerjee B, Chatrath H, Amirsolaimani B, Patel K, Patel C Biomed Opt Express. 2016; 7(1):148-57.

PMID: 26819824 PMC: 4722899. DOI: 10.1364/BOE.7.000148.

References
1.
Zhu C, Joyce N . Proliferative response of corneal endothelial cells from young and older donors. Invest Ophthalmol Vis Sci. 2004; 45(6):1743-51. DOI: 10.1167/iovs.03-0814. View

2.
Howat W, Wilson B . Tissue fixation and the effect of molecular fixatives on downstream staining procedures. Methods. 2014; 70(1):12-9. PMC: 4240801. DOI: 10.1016/j.ymeth.2014.01.022. View

3.
Boulze Pankert M, Goyer B, Zaguia F, Bareille M, Perron M, Liu X . Biocompatibility and functionality of a tissue-engineered living corneal stroma transplanted in the feline eye. Invest Ophthalmol Vis Sci. 2014; 55(10):6908-20. DOI: 10.1167/iovs.14-14720. View

4.
Aptel F, Olivier N, Deniset-Besseau A, Legeais J, Plamann K, Schanne-Klein M . Multimodal nonlinear imaging of the human cornea. Invest Ophthalmol Vis Sci. 2010; 51(5):2459-65. DOI: 10.1167/iovs.09-4586. View

5.
Vielreicher M, Schurmann S, Detsch R, Schmidt M, Buttgereit A, Boccaccini A . Taking a deep look: modern microscopy technologies to optimize the design and functionality of biocompatible scaffolds for tissue engineering in regenerative medicine. J R Soc Interface. 2013; 10(86):20130263. PMC: 3730680. DOI: 10.1098/rsif.2013.0263. View