The Antioxidant and Chemical Properties of Berberis Vulgaris and Its Cytotoxic Effect on Human Breast Carcinoma Cells

Overview

Journal Cytotechnology

Specialties Biotechnology
Genetics

Date 2015 Apr 29

PMID 25916942

Citations 14

Authors

Reyhane Hoshyar

Zahra Mahboob

Asghar Zarban

Affiliations

Soon will be listed here.

Abstract

In this study we evaluated the biological activity of alcoholic and aqueous extracts from the fruit of Berberis vulgaris. The total antioxidant capacity of Berberis was characterized by FRAP, DPPH, Folin-Ciocalteu while the anthocyanins content was measured by pH differential method. Cell viability and apoptotic property were determined by MTT and DNA fragmentation assays, respectively. Alcoholic extract of Berberis was richer in antioxidants and anthocyanins compared to aqueous extract. Although both extracts significantly inhibited proliferation of breast cancer cells (MCF-7); these changes were not observed in normal human breast epithelial cells (MCF10-A). The alcoholic extract was more effective in inducing apoptosis as detected by DNA fragmentation in treated cancer cells. Our results suggest that Berberis has potent antioxidant properties and cytotoxic effects that can induce apoptosis. Therefore, Berberis can potentially be exploited for the development of therapeutics to fight against human breast cancer.

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