The Inorganic Anatomy of the Mammalian Preimplantation Embryo and the Requirement of Zinc During the First Mitotic Divisions

Overview

Journal Dev Dyn

Publisher Wiley

Specialty Anatomy & Histology

Date 2015 Apr 24

PMID 25903945

Citations 19

Authors

Betty Y Kong

Francesca E Duncan

Emily L Que

Yuanming Xu

Stefan Vogt

Thomas V OHalloran

Teresa K Woodruff

Affiliations

Soon will be listed here.

Abstract

Background: Zinc is the most abundant transition metal in the mammalian oocyte, and dynamic fluxes in intracellular concentration are essential for regulating both meiotic progression and fertilization. Whether the defined pathways of zinc utilization in female meiosis directly translate to mitotic cells, including the mammalian preimplantation embryo, has not been studied previously.

Results: We determined that zinc is the most abundant transition metal in the preimplantation embryo, with levels an order of magnitude higher than those of iron or copper. Using a zinc-specific fluorescent probe, we demonstrated that labile zinc is distributed in vesicle-like structures in the cortex of cells at all stages of preimplantation embryo development. To test the importance of zinc during this period, we induced zinc insufficiency using the heavy metal chelator N,N,N',N'-tetrakis-(2-pyridylmethyl)-ethylenediamine (TPEN). Incubation of embryos in media containing TPEN resulted in a developmental arrest that was specific to zinc chelation and associated with compromised mitotic parameters. The developmental arrest due to zinc insufficiency was associated with altered chromatin structure in the blastomere nuclei and decreased global transcription.

Conclusions: These results demonstrate for the first time that the preimplantation embryo requires tight zinc regulation and homeostasis for the initial mitotic divisions of life.

Citing Articles

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Minerals and the Menstrual Cycle: Impacts on Ovulation and Endometrial Health.

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Preimplantation Developmental Competence of Bovine and Porcine Oocytes Activated by Zinc Chelation.

Cabeza J, Camera J, Briski O, Felipe M, Salamone D, Gambini A Animals (Basel). 2022; 12(24).

PMID: 36552480 PMC: 9774810. DOI: 10.3390/ani12243560.

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