Cytokine Networking of Chondrocyte Dedifferentiation in Vitro and Its Implications for Cell-based Cartilage Therapy

Overview

Journal Am J Transl Res

Specialty General Medicine

Date 2015 Apr 23

PMID 25901191

Citations 45

Authors

Li Duan

Bin Ma

Yujie Liang

Jielin Chen

Weimin Zhu

Mingtao Li

Daping Wang

Affiliations

Soon will be listed here.

Abstract

Autologous chondrocyte implantation (ACI) is a golden treatment for large defects of the knee joint without osteoarthritis or other complications. Despite notable progresses, generation of a stable chondrocyte phenotype using progenitor cells remains a main obstacle for chondrocyte-based cartilage treatment. Monolayer chondrocyte expansion in vitro is accompanied by chondrocyte dedifferentiation, which produces a non-specific mechanically inferior extracellular matrix (ECM) unsuitable for ACI. In-depth understanding of the molecular events during chondrocyte dedifferentiation is required to maintain the capacity of in vitro expanded chondrocytes to produce hyaline cartilage-specific ECM. This review discusses key cytokines and signaling pathways involved in chondrocyte dedifferentiation from the standpoint of catabolism and anabolism. Some potential therapeutic strategies are also presented to counteract chondrocyte dedifferentiation for cell-based cartilage therapy.

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References

Li C, Wang Q, Wang J . Transforming growth factor-β (TGF-β) induces the expression of chondrogenesis-related genes through TGF-β receptor II (TGFRII)-AKT-mTOR signaling in primary cultured mouse precartilaginous stem cells. Biochem Biophys Res Commun. 2014; 450(1):646-51. DOI: 10.1016/j.bbrc.2014.06.030. View

Galera P, Vivien D, Pronost S, Bonaventure J, Redini F, LOYAU G . Transforming growth factor-beta 1 (TGF-beta 1) up-regulation of collagen type II in primary cultures of rabbit articular chondrocytes (RAC) involves increased mRNA levels without affecting mRNA stability and procollagen processing. J Cell Physiol. 1992; 153(3):596-606. DOI: 10.1002/jcp.1041530322. View

Martin I, Vunjak-Novakovic G, Yang J, Langer R, Freed L . Mammalian chondrocytes expanded in the presence of fibroblast growth factor 2 maintain the ability to differentiate and regenerate three-dimensional cartilaginous tissue. Exp Cell Res. 1999; 253(2):681-8. DOI: 10.1006/excr.1999.4708. View

Hu W, Zhang W, Li F, Guo F, Chen A . Bortezomib prevents the expression of MMP-13 and the degradation of collagen type 2 in human chondrocytes. Biochem Biophys Res Commun. 2014; 452(3):526-30. DOI: 10.1016/j.bbrc.2014.08.102. View

Rottmar M, Mhanna R, Guimond-Lischer S, Vogel V, Zenobi-Wong M, Maniura-Weber K . Interference with the contractile machinery of the fibroblastic chondrocyte cytoskeleton induces re-expression of the cartilage phenotype through involvement of PI3K, PKC and MAPKs. Exp Cell Res. 2013; 320(2):175-87. DOI: 10.1016/j.yexcr.2013.11.004. View

Sylvester J, El Mabrouk M, Ahmad R, Chaudry A, Zafarullah M . Interleukin-1 induction of aggrecanase gene expression in human articular chondrocytes is mediated by mitogen-activated protein kinases. Cell Physiol Biochem. 2012; 30(3):563-74. DOI: 10.1159/000341438. View

Ashlin T, Kwan A, Ramji D . Regulation of ADAMTS-1, -4 and -5 expression in human macrophages: differential regulation by key cytokines implicated in atherosclerosis and novel synergism between TL1A and IL-17. Cytokine. 2013; 64(1):234-42. PMC: 3779352. DOI: 10.1016/j.cyto.2013.06.315. View

Blaise R, Mahjoub M, Salvat C, Barbe U, Brou C, Corvol M . Involvement of the Notch pathway in the regulation of matrix metalloproteinase 13 and the dedifferentiation of articular chondrocytes in murine cartilage. Arthritis Rheum. 2009; 60(2):428-39. DOI: 10.1002/art.24250. View

Groeneveld E, Burger E . Bone morphogenetic proteins in human bone regeneration. Eur J Endocrinol. 2000; 142(1):9-21. DOI: 10.1530/eje.0.1420009. View

10.

Kim D, Song J, Kim S, Park H, Chun C, Sonn J . MicroRNA-34a modulates cytoskeletal dynamics through regulating RhoA/Rac1 cross-talk in chondroblasts. J Biol Chem. 2012; 287(15):12501-9. PMC: 3320999. DOI: 10.1074/jbc.M111.264382. View

11.

Seifarth C, Csaki C, Shakibaei M . Anabolic actions of IGF-I and TGF-beta1 on Interleukin-1beta-treated human articular chondrocytes: evaluation in two and three dimensional cultures. Histol Histopathol. 2009; 24(10):1245-62. DOI: 10.14670/HH-24.1245. View

12.

Narcisi R, Quarto R, Ulivi V, Muraglia A, Molfetta L, Giannoni P . TGF β-1 administration during ex vivo expansion of human articular chondrocytes in a serum-free medium redirects the cell phenotype toward hypertrophy. J Cell Physiol. 2011; 227(9):3282-90. DOI: 10.1002/jcp.24024. View

13.

Lin Z, Fitzgerald J, Xu J, Willers C, Wood D, Grodzinsky A . Gene expression profiles of human chondrocytes during passaged monolayer cultivation. J Orthop Res. 2008; 26(9):1230-7. DOI: 10.1002/jor.20523. View

14.

Geesink R, Hoefnagels N, Bulstra S . Osteogenic activity of OP-1 bone morphogenetic protein (BMP-7) in a human fibular defect. J Bone Joint Surg Br. 1999; 81(4):710-8. DOI: 10.1302/0301-620x.81b4.9311. View

15.

Goldring M, Otero M, Tsuchimochi K, Ijiri K, Li Y . Defining the roles of inflammatory and anabolic cytokines in cartilage metabolism. Ann Rheum Dis. 2008; 67 Suppl 3:iii75-82. PMC: 3939701. DOI: 10.1136/ard.2008.098764. View

16.

Miyazaki Y, Tsukazaki T, Hirota Y, Yonekura A, Osaki M, Shindo H . Dexamethasone inhibition of TGF beta-induced cell growth and type II collagen mRNA expression through ERK-integrated AP-1 activity in cultured rat articular chondrocytes. Osteoarthritis Cartilage. 2000; 8(5):378-85. DOI: 10.1053/joca.1999.0313. View

17.

Lohmann C, Schwartz Z, Niederauer G, Boyan B . [Degree of differentiation of chondrocytes and their pretreatment with platelet-derived-growth factor. Regulating induction of cartilage formation in resorbable tissue carriers in vivo]. Orthopade. 2000; 29(2):120-8. DOI: 10.1007/s001320050020. View

18.

Thomas B, Thirion S, Humbert L, Tan L, Goldring M, Bereziat G . Differentiation regulates interleukin-1beta-induced cyclo-oxygenase-2 in human articular chondrocytes: role of p38 mitogen-activated protein kinase. Biochem J. 2002; 362(Pt 2):367-73. PMC: 1222396. DOI: 10.1042/0264-6021:3620367. View

19.

Friedlaender G, Perry C, Cole J, Cook S, Cierny G, Muschler G . Osteogenic protein-1 (bone morphogenetic protein-7) in the treatment of tibial nonunions. J Bone Joint Surg Am. 2001; 83-A Suppl 1(Pt 2):S151-8. PMC: 1425155. View

20.

Goldring M, Birkhead J, Sandell L, Kimura T, Krane S . Interleukin 1 suppresses expression of cartilage-specific types II and IX collagens and increases types I and III collagens in human chondrocytes. J Clin Invest. 1988; 82(6):2026-37. PMC: 442785. DOI: 10.1172/JCI113823. View