The Effects of Amlodipine and Platelet Rich Plasma on Bone Healing in Rats

Overview

Journal Drug Des Devel Ther

Specialty Pharmacology

Date 2015 Apr 22

PMID 25897207

Citations 5

Authors

Yusuf Atalay

Mehmet Fatih Bozkurt

Yucel Gonul

Omer Cakmak

Kamil Serkan Agacayak

Ibrahim Kose

Omer Hazman

Hikmet Keles

Ozan Turamanlar

Mehmet Eroglu

Affiliations

Soon will be listed here.

Abstract

Aim: The aim of this study was to evaluate the effects of calcium channel blocker (CCB) amlodipine (AML), platelet rich plasma (PRP), and a mixture of both materials on bone healing.

Materials And Methods: Fifty-six male Wistar rats were randomly divided into four groups: group A, tibia defect model with no treatment; group B, tibia defect model treated with AML, 0.04 mg daily by oral gavage; group C, tibia defect model treated with local PRP; group D, tibia defect model treated with local PRP and AML, 0.04 mg daily by oral gavage.

Results: At day 21, bone healing was significantly better in groups C and D compared to group A (P<0.05), but comparisons showed no statistically significant difference in group B (P>0.05). At day 30, groups B and C showed no statistically significant difference (P>0.05) compared to group A, but bone healing in group D was significantly better than in group A (P<0.05). Statistically, AML did not affect alkaline phosphatase (ALP) activity at 21 and 30 days (P>0.05), but PRP and AML + PRP increased ALP activity statistically (P<0.05).

Conclusion: It can be concluded that AML had neither a positive nor a negative effect on bone healing, but when used in combination with PRP, it may be beneficial.

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References

Alsousou J, Thompson M, Hulley P, Noble A, Willett K . The biology of platelet-rich plasma and its application in trauma and orthopaedic surgery: a review of the literature. J Bone Joint Surg Br. 2009; 91(8):987-96. DOI: 10.1302/0301-620X.91B8.22546. View

Piemontese M, Aspriello S, Rubini C, Ferrante L, Procaccini M . Treatment of periodontal intrabony defects with demineralized freeze-dried bone allograft in combination with platelet-rich plasma: a comparative clinical trial. J Periodontol. 2008; 79(5):802-10. DOI: 10.1902/jop.2008.070436. View

Whitman D, Berry R, Green D . Platelet gel: an autologous alternative to fibrin glue with applications in oral and maxillofacial surgery. J Oral Maxillofac Surg. 1997; 55(11):1294-9. DOI: 10.1016/s0278-2391(97)90187-7. View

Fabbro M, Bortolin M, Taschieri S . Is autologous platelet concentrate beneficial for post-extraction socket healing? A systematic review. Int J Oral Maxillofac Surg. 2011; 40(9):891-900. DOI: 10.1016/j.ijom.2011.04.009. View

Anitua E, Prado R, Orive G . Bilateral sinus elevation evaluating plasma rich in growth factors technology: a report of five cases. Clin Implant Dent Relat Res. 2010; 14(1):51-60. DOI: 10.1111/j.1708-8208.2009.00233.x. View

Kasten P, Luginbuhl R, van Griensven M, Barkhausen T, Krettek C, Bohner M . Comparison of human bone marrow stromal cells seeded on calcium-deficient hydroxyapatite, beta-tricalcium phosphate and demineralized bone matrix. Biomaterials. 2003; 24(15):2593-603. DOI: 10.1016/s0142-9612(03)00062-0. View

Moraes R, Correa L, Cerqueira Luz J . Adverse effects of the amlodipine on bone healing of the mandibular fracture: an experimental study in rats. Oral Maxillofac Surg. 2010; 15(2):93-101. DOI: 10.1007/s10006-010-0237-6. View

Weibrich G, Kleis W, Hafner G . Growth factor levels in the platelet-rich plasma produced by 2 different methods: curasan-type PRP kit versus PCCS PRP system. Int J Oral Maxillofac Implants. 2002; 17(2):184-90. View

Gu Y, Preston M, Magnay J, El Haj A, Publicover S . Hormonally-regulated expression of voltage-operated Ca(2+) channels in osteocytic (MLO-Y4) cells. Biochem Biophys Res Commun. 2001; 282(2):536-42. DOI: 10.1006/bbrc.2001.4615. View

10.

Jensen T, Rahbek O, Overgaard S, Soballe K . Platelet rich plasma and fresh frozen bone allograft as enhancement of implant fixation. An experimental study in dogs. J Orthop Res. 2004; 22(3):653-8. DOI: 10.1016/j.orthres.2003.10.006. View

11.

Daif E . Effect of autologous platelet-rich plasma on bone regeneration in mandibular fractures. Dent Traumatol. 2012; 29(5):399-403. DOI: 10.1111/edt.12021. View

12.

. 1999 World Health Organization-International Society of Hypertension Guidelines for the Management of Hypertension. Guidelines Subcommittee. J Hypertens. 1999; 17(2):151-83. View

13.

Marx R, Carlson E, Eichstaedt R, Schimmele S, Strauss J, Georgeff K . Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998; 85(6):638-46. DOI: 10.1016/s1079-2104(98)90029-4. View

14.

Anitua E . Plasma rich in growth factors: preliminary results of use in the preparation of future sites for implants. Int J Oral Maxillofac Implants. 1999; 14(4):529-35. View

15.

Chen T, Lu H, Liu G, Tang D, Zhang X, Pan Z . Effect of autologous platelet-rich plasma in combination with bovine porous bone mineral and bio-guide membrane on bone regeneration in mandible bicortical bony defects. J Craniofac Surg. 2014; 25(1):215-23. DOI: 10.1097/SCS.0000000000000420. View

16.

Anitua E, Andia I, Ardanza B, Nurden P, Nurden A . Autologous platelets as a source of proteins for healing and tissue regeneration. Thromb Haemost. 2003; 91(1):4-15. DOI: 10.1160/TH03-07-0440. View

17.

Ushijima K, Liu Y, Maekawa T, Ishikawa E, Motosugi Y, Ando H . Protective effect of amlodipine against osteoporosis in stroke-prone spontaneously hypertensive rats. Eur J Pharmacol. 2010; 635(1-3):227-30. DOI: 10.1016/j.ejphar.2010.02.039. View

18.

Boyapati L, Wang H . The role of platelet-rich plasma in sinus augmentation: a critical review. Implant Dent. 2006; 15(2):160-70. DOI: 10.1097/01.id.0000217791.74343.60. View

19.

Kisiel A, McDuffee L, Masaoud E, Bailey T, Esparza Gonzalez B, Nino-Fong R . Isolation, characterization, and in vitro proliferation of canine mesenchymal stem cells derived from bone marrow, adipose tissue, muscle, and periosteum. Am J Vet Res. 2012; 73(8):1305-17. DOI: 10.2460/ajvr.73.8.1305. View

20.

Lubowitz J, Poehling G . Shoulder, hip, knee, and PRP. Arthroscopy. 2010; 26(2):141-2. DOI: 10.1016/j.arthro.2009.12.005. View