MiR-20b is Up-regulated in Brain Metastases from Primary Breast Cancers

Overview

Journal Oncotarget

Specialty Oncology

Date 2015 Apr 21

PMID 25893380

Citations 35

Authors

Aamir Ahmad

Kevin R Ginnebaugh

Seema Sethi

Wei Chen

Rouba Ali

Sandeep Mittal

Fazlul H Sarkar

Affiliations

Soon will be listed here.

Abstract

Brain metastases are frequent in patients with advanced breast cancer and are associated with poor prognosis. However, unique molecular biomarkers have not yet been established. We hypothesized that microRNA-20b (miR-20b) plays a role in breast cancer brain metastasis. Our study cohort comprised of eleven breast cancer patients with brain metastasis and nine control patients (age, stage, and follow-up matched) with breast cancer without brain metastasis. Cases were reviewed microscopically to select tumor blocks with >50% tumor cells, RNA was extracted from formalin-fixed paraffin-embedded (FFPE) tumor tissue blocks and expression of miR-20b analyzed using qRT-PCR. We further tested the effect of miR-20b overexpression on colony formation and invasion in vitro using MCF-7 and MDA-MB-231 cells. In the patient-derived samples, miR-20b expression was significantly higher in brain metastases of breast cancer patients, compared to primary breast tumors as well as the patients without brain metastasis. miR-20b also significantly induced the colony formation and invasiveness of breast cancer cells. Further, miR-20b levels were observed to be high in brain-metastasizing cells, compared to bone-metastasizing cells. Together, our findings suggest a novel role of miR-20b in breast cancer brain metastasis that warrants further investigation for its potential to be developed as prognostic and/or therapeutic target.

Citing Articles

Crosstalk between breast cancer-derived microRNAs and brain microenvironmental cells in breast cancer brain metastasis.

Khan M, Wong G, Zhuang C, Najjar M, Lo H Front Oncol. 2024; 14:1436942.

PMID: 39175471 PMC: 11338853. DOI: 10.3389/fonc.2024.1436942.

MiRNAs as new potential biomarkers and therapeutic targets in brain metastasis.

Beylerli O, Shi H, Begliarzade S, Shumadalova A, Ilyasova T, Sufianov A Noncoding RNA Res. 2024; 9(3):678-686.

PMID: 38577014 PMC: 10987301. DOI: 10.1016/j.ncrna.2024.02.014.

New insights of miRNA molecular mechanisms in breast cancer brain metastasis and therapeutic targets.

Hussen B, Abdullah K, Abdullah S, Majeed N, Mohamadtahr S, Rasul M Noncoding RNA Res. 2023; 8(4):645-660.

PMID: 37818447 PMC: 10560790. DOI: 10.1016/j.ncrna.2023.09.003.

Advances in Brain Metastases Diagnosis: Non-coding RNAs As Potential Biomarkers.

Eraky A Cureus. 2023; 15(3):e36337.

PMID: 37077610 PMC: 10109215. DOI: 10.7759/cureus.36337.

Effect of Silibinin on the Expression of Mir-20b, Bcl2L11, and Erbb2 in Breast Cancer Cell Lines.

Ranapour S, Motamed N Mol Biotechnol. 2023; 65(12):1979-1990.

PMID: 36905464 DOI: 10.1007/s12033-023-00702-5.

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