The Effects of Multifunctional MiR-122-loaded Graphene-gold Composites on Drug-resistant Liver Cancer

Overview

Journal J Nanobiotechnology

Publisher Biomed Central

Specialty Biotechnology

Date 2015 Apr 18

PMID 25885701

Citations 15

Authors

Yi Yuan

Yaqin Zhang

Bin Liu

Heming Wu

Yanjun Kang

Ming Li

Xin Zeng

Nongyue He

Gen Zhang

Affiliations

Soon will be listed here.

Abstract

Background: Nano drugs have attracted increased attention due to their unique mode of action that offers tumor-inhibiting effects. Therefore, we have previously explored functionalized and drug-loaded graphene-gold nanocomposites that induced cancer cell apoptosis.

Results: In the present study, we developed a combination of monoclonal P-glycoprotein (P-gp) antibodies, folic acid (FA) and miR-122-loaded gold nanoparticles on graphene nanocomposites (GGMPN), which promoted drug-resistant HepG2 cell apoptosis with drug targeting and controlled release properties. We also investigated related apoptosis proteins and apoptosis signal pathways by GGMPN treatment in vitro and in vivo. Moreover, we further demonstrated the inhibition of tumor growth and the apoptosis-inducing effect by means of GGMPN with a semiconductor laser in a xenograft tumor model.

Conclusion: In conclusion, our results collectively suggested that GGMPN could serve as a novel therapeutic approach to control tumor cell apoptosis and growth.

Citing Articles

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MicroRNA-122 in human cancers: from mechanistic to clinical perspectives.

Faramin Lashkarian M, Hashemipour N, Niaraki N, Soghala S, Moradi A, Sarhangi S Cancer Cell Int. 2023; 23(1):29.

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Gold Nanoconjugates for miRNA Modulation in Cancer Therapy: From miRNA Silencing to miRNA Mimics.

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Functionalized Graphene Oxide for Chemotherapeutic Drug Delivery and Cancer Treatment: A Promising Material in Nanomedicine.

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References

Hayama A, Yamamoto T, Yokoyama M, Kawano K, Hattori Y, Maitani Y . Polymeric micelles modified by folate-PEG-lipid for targeted drug delivery to cancer cells in vitro. J Nanosci Nanotechnol. 2008; 8(6):3085-90. DOI: 10.1166/jnn.2008.093. View

Gui C, Cui D . Functionalized gold nanorods for tumor imaging and targeted therapy. Cancer Biol Med. 2013; 9(4):221-33. PMC: 3643678. DOI: 10.7497/j.issn.2095-3941.2012.04.002. View

Pathak A, Vyas S, Gupta K . Nano-vectors for efficient liver specific gene transfer. Int J Nanomedicine. 2008; 3(1):31-49. PMC: 2526359. View

Hou Z, Zhan C, Jiang Q, Hu Q, Li L, Chang D . Both FA- and mPEG-conjugated chitosan nanoparticles for targeted cellular uptake and enhanced tumor tissue distribution. Nanoscale Res Lett. 2011; 6(1):563. PMC: 3306018. DOI: 10.1186/1556-276X-6-563. View

Basu S, Bhattacharyya S . Insulin-like growth factor-1 prevents miR-122 production in neighbouring cells to curtail its intercellular transfer to ensure proliferation of human hepatoma cells. Nucleic Acids Res. 2014; 42(11):7170-85. PMC: 4066773. DOI: 10.1093/nar/gku346. View

Solarska K, Gajewska A, Bartosz G, Mitura K . Induction of apoptosis in human endothelial cells by nanodiamond particles. J Nanosci Nanotechnol. 2012; 12(6):5117-21. DOI: 10.1166/jnn.2012.4952. View

Li W, Abu Samra D, Merzaban J, Khashab N . P-glycoprotein targeted nanoscale drug carriers. J Nanosci Nanotechnol. 2013; 13(2):1399-402. DOI: 10.1166/jnn.2013.6084. View

van Vlerken L, Amiji M . Multi-functional polymeric nanoparticles for tumour-targeted drug delivery. Expert Opin Drug Deliv. 2006; 3(2):205-16. DOI: 10.1517/17425247.3.2.205. View

Bellamy W . P-glycoproteins and multidrug resistance. Annu Rev Pharmacol Toxicol. 1996; 36:161-83. DOI: 10.1146/annurev.pa.36.040196.001113. View

10.

Sahu A, Choi W, Lee J, Tae G . Graphene oxide mediated delivery of methylene blue for combined photodynamic and photothermal therapy. Biomaterials. 2013; 34(26):6239-48. DOI: 10.1016/j.biomaterials.2013.04.066. View

11.

McGuire J, Haile W, Yeh C . 5-amino-4-imidazolecarboxamide riboside potentiates both transport of reduced folates and antifolates by the human reduced folate carrier and their subsequent metabolism. Cancer Res. 2006; 66(7):3836-44. DOI: 10.1158/0008-5472.CAN-05-3226. View

12.

Chiappetta D, Sosnik A . Poly(ethylene oxide)-poly(propylene oxide) block copolymer micelles as drug delivery agents: improved hydrosolubility, stability and bioavailability of drugs. Eur J Pharm Biopharm. 2007; 66(3):303-17. DOI: 10.1016/j.ejpb.2007.03.022. View

13.

Zhao R, Hanscom M, Chattopadhyay S, Goldman I . Selective preservation of pemetrexed pharmacological activity in HeLa cells lacking the reduced folate carrier: association with the presence of a secondary transport pathway. Cancer Res. 2004; 64(9):3313-9. DOI: 10.1158/0008-5472.can-03-3953. View

14.

Lin C, Gong H, Tseng H, Wang W, Wu J . miR-122 targets an anti-apoptotic gene, Bcl-w, in human hepatocellular carcinoma cell lines. Biochem Biophys Res Commun. 2008; 375(3):315-20. DOI: 10.1016/j.bbrc.2008.07.154. View

15.

Long Q, Xiel Y, Huang Y, Wu Q, Zhang H, Xiong S . Induction of apoptosis and inhibition of angiogenesis by PEGylated liposomal quercetin in both cisplatin-sensitive and cisplatin-resistant ovarian cancers. J Biomed Nanotechnol. 2013; 9(6):965-75. DOI: 10.1166/jbn.2013.1596. View

16.

Filipowicz W, Grosshans H . The liver-specific microRNA miR-122: biology and therapeutic potential. Prog Drug Res. 2010; 67:221-38. View

17.

Ruan J, Song H, Li C, Bao C, Fu H, Wang K . DiR-labeled Embryonic Stem Cells for Targeted Imaging of in vivo Gastric Cancer Cells. Theranostics. 2012; 2(6):618-28. PMC: 3388594. DOI: 10.7150/thno.4561. View

18.

Hou W, Bukong T, Kodys K, Szabo G . Alcohol facilitates HCV RNA replication via up-regulation of miR-122 expression and inhibition of cyclin G1 in human hepatoma cells. Alcohol Clin Exp Res. 2012; 37(4):599-608. PMC: 4171687. DOI: 10.1111/acer.12005. View

19.

Khlebtsov N, Bogatyrev V, Dykman L, Khlebtsov B, Staroverov S, Shirokov A . Analytical and theranostic applications of gold nanoparticles and multifunctional nanocomposites. Theranostics. 2013; 3(3):167-80. PMC: 3590586. DOI: 10.7150/thno.5716. View

20.

Stark G . Cancer chemotherapy. Progress in understanding multidrug resistance. Nature. 1986; 324(6096):407-8. DOI: 10.1038/324407a0. View