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Optimized Protocol for Simple Extraction of High-Quality Genomic DNA from Clostridium Difficile for Whole-Genome Sequencing

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 2015 Apr 17
PMID 25878343
Citations 5
Authors
James Heng Chiak Sim
Victoria Anikst
Akshar Lohith
Nader Pourmand
Niaz Banaei
Affiliations
Soon will be listed here.
Abstract

Successful sequencing of the Clostridium difficile genome requires high-quality genomic DNA (gDNA) as the starting material. gDNA extraction using conventional methods is laborious. We describe here an optimized method for the simple extraction of C. difficile gDNA using the QIAamp DNA minikit, which yielded high-quality sequence reads on the Illumina MiSeq platform.

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