Anuran Trypanosomes: Phylogenetic Evidence for New Clades in Brazil

Overview

Journal Syst Parasitol

Specialty Parasitology

Date 2015 Apr 12

PMID 25862033

Citations 8

Authors

Juliana I G da S Ferreira

Andrea P da Costa

Diego Ramirez

Jairo A M Roldan

Danilo Saraiva

Gislene F R da S Founier

Ana Sue

Erick R Zambelli

Antonio H H Minervino

Vanessa K Verdade

Solange M Gennari

Arlei Marcili

Affiliations

Soon will be listed here.

Abstract

Trypanosomes of anurans and fish are grouped into the Aquatic Clade which includes species isolated from fish, amphibians, turtles and platypus, usually transmitted by leeches and phlebotomine sand flies. Trypanosomes from Brazilian frogs are grouped within the Aquatic Clade with other anuran trypanosome species, where there seems to be coevolutionary patterns with vertebrate hosts and association to Brazilian biomes (Atlantic Forest, Pantanal and Amazonia Rainforest). We characterised the anuran trypanosomes from two different areas of the Cerrado biome and examined their phylogenetic relationships based on the SSU rRNA gene. A total of 112 anurans of six species was analysed and trypanosome prevalence evaluated through haemoculture was found to be 7% (8 positive frogs). However, only three isolates (2.7%) from two anuran species were recovered and cryopreserved. Analysis including SSU rDNA sequences from previous studies segregated the anuran trypanosomes into six groups, the previously reported An01 to An04, and An05 and An06 reported herein. Clade An05 comprises the isolates from Leptodactylus latrans (Steffen) and Pristimantis sp. captured in the Cerrado biome and Trypanosoma chattoni Mathis & Leger, 1911. The inclusion of new isolates in the phylogenetic analyses provided evidence for a new group (An06) of parasites from phlebotomine hosts. Our results indicate that the diversity of trypanosome species is underestimated since studies conducted in Brazil and other regions of the world are still few.

Citing Articles

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References

Martin D, Wright A, Barta J, Desser S . Phylogenetic position of the giant anuran trypanosomes Trypanosoma chattoni, Trypanosoma fallisi, Trypanosoma mega, Trypanosoma neveulemairei, and Trypanosoma ranarum inferred from 18S rRNA gene sequences. J Parasitol. 2002; 88(3):566-71. DOI: 10.1645/0022-3395(2002)088[0566:PPOTGA]2.0.CO;2. View

Camargo E . GROWTH AND DIFFERENTIATION IN TRYPANOSOMA CRUZI. I. ORIGIN OF METACYCLIC TRYPANOSOMES IN LIQUID MEDIA. Rev Inst Med Trop Sao Paulo. 1964; 6:93-100. View

Hamilton P, Stevens J, Gaunt M, Gidley J, Gibson W . Trypanosomes are monophyletic: evidence from genes for glyceraldehyde phosphate dehydrogenase and small subunit ribosomal RNA. Int J Parasitol. 2004; 34(12):1393-404. DOI: 10.1016/j.ijpara.2004.08.011. View

Buckley L, Jetz W . Environmental and historical constraints on global patterns of amphibian richness. Proc Biol Sci. 2007; 274(1614):1167-73. PMC: 2189569. DOI: 10.1098/rspb.2006.0436. View

Paparini A, MacGregor J, Irwin P, Warren K, Ryan U . Novel genotypes of Trypanosoma binneyi from wild platypuses (Ornithorhynchus anatinus) and identification of a leech as a potential vector. Exp Parasitol. 2014; 145:42-50. DOI: 10.1016/j.exppara.2014.07.004. View

Ferreira R, De Souza A, Freitas R, Campaner M, Takata C, Barrett T . A phylogenetic lineage of closely related trypanosomes (Trypanosomatidae, Kinetoplastida) of anurans and sand flies (Psychodidae, Diptera) sharing the same ecotopes in brazilian amazonia. J Eukaryot Microbiol. 2008; 55(5):427-35. DOI: 10.1111/j.1550-7408.2008.00342.x. View

Simpson A, Stevens J, Lukes J . The evolution and diversity of kinetoplastid flagellates. Trends Parasitol. 2006; 22(4):168-74. DOI: 10.1016/j.pt.2006.02.006. View

Vickerman K . The evolutionary expansion of the trypanosomatid flagellates. Int J Parasitol. 1994; 24(8):1317-31. DOI: 10.1016/0020-7519(94)90198-8. View

Viola L, Attias M, Takata C, Campaner M, De Souza W, Camargo E . Phylogenetic analyses based on small subunit rRNA and glycosomal glyceraldehyde-3-phosphate dehydrogenase genes and ultrastructural characterization of two snake Trypanosomes: Trypanosoma serpentis n. sp. from Pseudoboa nigra and Trypanosoma.... J Eukaryot Microbiol. 2009; 56(6):594-602. DOI: 10.1111/j.1550-7408.2009.00444.x. View

10.

Lemos M, Morais D, Carvalho V, DAgosto M . First record of Trypanosoma chattoni in Brazil and occurrence of other Trypanosoma species in Brazilian frogs (Anura, Leptodactylidae). J Parasitol. 2008; 94(1):148-51. DOI: 10.1645/GE-1095.1. View

11.

Thompson J, Gibson T, Plewniak F, Jeanmougin F, Higgins D . The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1998; 25(24):4876-82. PMC: 147148. DOI: 10.1093/nar/25.24.4876. View

12.

Cavalier-Smith T . Only six kingdoms of life. Proc Biol Sci. 2004; 271(1545):1251-62. PMC: 1691724. DOI: 10.1098/rspb.2004.2705. View

13.

Viola L, Almeida R, Ferreira R, Campaner M, Takata C, Rodrigues A . Evolutionary history of trypanosomes from South American caiman (Caiman yacare) and African crocodiles inferred by phylogenetic analyses using SSU rDNA and gGAPDH genes. Parasitology. 2008; 136(1):55-65. DOI: 10.1017/S003118200800512X. View

14.

Maia da Silva F, Marcili A, Lima L, Cavazzana Jr M, Ortiz P, Campaner M . Trypanosoma rangeli isolates of bats from Central Brazil: genotyping and phylogenetic analysis enable description of a new lineage using spliced-leader gene sequences. Acta Trop. 2008; 109(3):199-207. DOI: 10.1016/j.actatropica.2008.11.005. View

15.

Bardsley J, Harmsen R . The trypanosomes of anura. Adv Parasitol. 1973; 11(0):1-73. DOI: 10.1016/s0065-308x(08)60184-0. View

16.

Hamilton P, Gibson W, Stevens J . Patterns of co-evolution between trypanosomes and their hosts deduced from ribosomal RNA and protein-coding gene phylogenies. Mol Phylogenet Evol. 2007; 44(1):15-25. DOI: 10.1016/j.ympev.2007.03.023. View

17.

Desser S . The blood parasites of anurans from Costa Rica with reflections on the taxonomy of their trypanosomes. J Parasitol. 2001; 87(1):152-60. DOI: 10.1645/0022-3395(2001)087[0152:TBPOAF]2.0.CO;2. View

18.

Marcili A, da Costa A, Soares H, da C L Acosta I, de Lima J, Minervino A . Isolation and phylogenetic relationships of bat trypanosomes from different biomes in Mato Grosso, Brazil. J Parasitol. 2013; 99(6):1071-6. DOI: 10.1645/12-156.1. View

19.

Huelsenbeck J, Ronquist F . MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics. 2001; 17(8):754-5. DOI: 10.1093/bioinformatics/17.8.754. View

20.

Hamilton P, Stevens J, Gidley J, Holz P, Gibson W . A new lineage of trypanosomes from Australian vertebrates and terrestrial bloodsucking leeches (Haemadipsidae). Int J Parasitol. 2005; 35(4):431-43. DOI: 10.1016/j.ijpara.2004.12.005. View