Epigenetic Mechanisms Contribute to the Expression of Immune Related Genes in the Livers of Dairy Cows Fed a High Concentrate Diet

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2015 Apr 11

PMID 25860644

Citations 9

Authors

Guangjun Chang

Kai Zhang

Tianle Xu

Di Jin

Junfei Guo

Su Zhuang

Xiangzhen Shen

Affiliations

Soon will be listed here.

Abstract

Purpose: Epigenetic modifications critically regulate the expression of immune-related genes in response to inflammatory stimuli. It has been extensively reported that a high concentrate (HC) diet can trigger systemic inflammation in dairy cows, yet it is unclear whether epigenetic regulation is involved in the expression of immune genes in the livers of dairy cows. This study aimed to investigate the impact of epigenetic modifications on the expression of immune-related genes.

Experimental Design: In eight mid-lactating cows, we installed a rumen cannula and catheters of the portal and hepatic veins. Cows were randomly assigned to either the treatment group fed a high concentrate (HC) diet (60% concentrate + 40% forage, n = 4) or a control group fed a low concentrate (LC) diet (40% concentrate + 60% forage, n = 4).

Results: After 10 weeks of feeding, the rumen pH was reduced, and levels of lipopolysaccharide (LPS) in the rumen, and portal and hepatic veins were notably increased in the HC group compared with the LC group. The expression levels of detected immune response-related genes, including Toll-like receptor 4 (TLR4), cytokines, chemokines, and acute phase proteins, were significantly up-regulated in the livers of cows fed a HC diet. Chromatin loosening at the promoter region of four candidate immune-related genes (TLR4, LPS-binding protein, haptoglobin, and serum amyloid A3) was elicited, and was strongly correlated with enhanced expression of these genes in the HC group. Demethylation at the promoter region of all four candidate immune-related genes was accompanied by chromatin decompaction.

Conclusion: After HC diet feeding, LPS derived from the digestive tract translocated to the liver via the portal vein, enhancing hepatic immune gene expression. The up-regulation of these immune genes was mediated by epigenetic mechanisms, which involve chromatin remodeling and DNA methylation. Our findings suggest that modulating epigenetic mechanisms could provide novel ways to treat systemic inflammatory responses elicited by the feeding of a HC diet.

Citing Articles

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Paraclinical Changes Occurring in Dairy Cows with Spontaneous Subacute Ruminal Acidosis under Field Conditions.

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Impacts of Epigenetic Processes on the Health and Productivity of Livestock.

Wang M, Ibeagha-Awemu E Front Genet. 2021; 11:613636.

PMID: 33708235 PMC: 7942785. DOI: 10.3389/fgene.2020.613636.

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