Antibiotic-free Selection in Biotherapeutics: Now and Forever

Overview

Journal Pathogens

Date 2015 Apr 10

PMID 25854922

Citations 40

Authors

Charlotte Mignon

Regis Sodoyer

Bettina Werle

Affiliations

Soon will be listed here.

Abstract

The continuously improving sophistication of molecular engineering techniques gives access to novel classes of bio-therapeutics and new challenges for their production in full respect of the strengthening regulations. Among these biologic agents are DNA based vaccines or gene therapy products and to a lesser extent genetically engineered live vaccines or delivery vehicles. The use of antibiotic-based selection, frequently associated with genetic manipulation of microorganism is currently undergoing a profound metamorphosis with the implementation and diversification of alternative selection means. This short review will present examples of alternatives to antibiotic selection and their context of application to highlight their ineluctable invasion of the bio-therapeutic world.

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