Serum Antioxidant Enzymes Activities and Oxidative Stress Levels in Patients with Acute Ischemic Stroke: Influence on Neurological Status and Outcome

Overview

Journal Wien Klin Wochenschr

Publisher Springer

Specialty General Medicine

Date 2015 Apr 10

PMID 25854910

Citations 25

Authors

Aysel Milanlioglu

Mehmet Aslan

Halil Ozkol

Vedat Cilingir

Mehmet Nuri Aydin

Sevdegul Karadas

Affiliations

Soon will be listed here.

Abstract

Background: Oxidative stress is well believed to play a role in the pathogenesis of acute ischemic stroke. Reports on antioxidant enzyme activities in patients with stroke are conflicting. Therefore, the aim of this study was to investigate serum antioxidant enzyme activities and oxidative stress levels in patients with acute ischemic stroke within 1st, 5th, and 21st day after stroke onset and also the relationship between these results and the clinical status of patients.

Methods: The current study comprised 45 patients with acute ischemic stroke and 30 healthy controls. Serum malondialdehyde (MDA) levels, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase activities were measured spectrophotometrically.

Results: Serum MDA levels were significantly higher in acute ischemic stroke patients within 24 h after stroke onset than controls (p < 0.05), whereas serum catalase activity was significantly lower (p < 0.05). There were no significant differences in GSH-Px and SOD activities. Serum catalase and SOD activities were significantly lower in fifth day than those of controls (both, p < 0.05) but GSH-Px activity and MDA levels did not change (p > 0.05). Serum SOD activity was significantly lower in 21st day compared to SOD activity of controls (p < 0.05) but MDA levels, GSH-Px, and CAT activities did not change significantly.

Conclusions: Our study demonstrated that acute ischemic stroke patients have increased oxidative stress and decreased antioxidant enzymes activities. These findings indicated that an imbalance of oxidant and antioxidant status might play a role in the pathogenesis of acute ischemic stroke.

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References

Cherubini A, Polidori M, Bregnocchi M, Pezzuto S, Cecchetti R, Ingegni T . Antioxidant profile and early outcome in stroke patients. Stroke. 2000; 31(10):2295-300. DOI: 10.1161/01.str.31.10.2295. View

El Kossi M, Zakhary M . Oxidative stress in the context of acute cerebrovascular stroke. Stroke. 2000; 31(8):1889-92. DOI: 10.1161/01.str.31.8.1889. View

Cojocaru I, Cojocaru M, Sapira V, Ionescu A . Evaluation of oxidative stress in patients with acute ischemic stroke. Rom J Intern Med. 2013; 51(2):97-106. View

Alonso de Lecinana M, Egido J, Fernandez C, Martinez-Vila E, Santos S, Morales A . Risk of ischemic stroke and lifetime estrogen exposure. Neurology. 2007; 68(1):33-8. DOI: 10.1212/01.wnl.0000250238.69938.f5. View

Adams Jr H, Bendixen B, Kappelle L, Biller J, Love B, Gordon D . Classification of subtype of acute ischemic stroke. Definitions for use in a multicenter clinical trial. TOAST. Trial of Org 10172 in Acute Stroke Treatment. Stroke. 1993; 24(1):35-41. DOI: 10.1161/01.str.24.1.35. View

Ames B, Shigenaga M, Hagen T . Oxidants, antioxidants, and the degenerative diseases of aging. Proc Natl Acad Sci U S A. 1993; 90(17):7915-22. PMC: 47258. DOI: 10.1073/pnas.90.17.7915. View

Yousuf S, Atif F, Ahmad M, Hoda M, Khan M, Ishrat T . Selenium plays a modulatory role against cerebral ischemia-induced neuronal damage in rat hippocampus. Brain Res. 2007; 1147:218-25. DOI: 10.1016/j.brainres.2007.01.143. View

Spranger M, Krempien S, Schwab S, Donneberg S, Hacke W . Superoxide dismutase activity in serum of patients with acute cerebral ischemic injury. Correlation with clinical course and infarct size. Stroke. 1997; 28(12):2425-8. DOI: 10.1161/01.str.28.12.2425. View

Demirkaya S, Topcuoglu M, Aydin A, Ulas U, Isimer A, Vural O . Malondialdehyde, glutathione peroxidase and superoxide dismutase in peripheral blood erythrocytes of patients with acute cerebral ischemia. Eur J Neurol. 2001; 8(1):43-51. DOI: 10.1046/j.1468-1331.2001.00166.x. View

10.

Moro M, Almeida A, Bolanos J, Lizasoain I . Mitochondrial respiratory chain and free radical generation in stroke. Free Radic Biol Med. 2005; 39(10):1291-304. DOI: 10.1016/j.freeradbiomed.2005.07.010. View

11.

Karahocagil M, Aslan M, Ceylan M, Cikman A, Sunnetcioglu M, Kucukoglu M . Serum myeloperoxidase activity and oxidative stress in patients with acute brucellosis. Clin Biochem. 2012; 45(10-11):733-6. DOI: 10.1016/j.clinbiochem.2012.03.017. View

12.

Aygul R, Kotan D, Demirbas F, Ulvi H, Deniz O . Plasma oxidants and antioxidants in acute ischaemic stroke. J Int Med Res. 2006; 34(4):413-8. DOI: 10.1177/147323000603400411. View

13.

Wang X, Michaelis E . Selective neuronal vulnerability to oxidative stress in the brain. Front Aging Neurosci. 2010; 2:12. PMC: 2874397. DOI: 10.3389/fnagi.2010.00012. View

14.

Ozkul A, Akyol A, Yenisey C, Arpaci E, Kiylioglu N, Tataroglu C . Oxidative stress in acute ischemic stroke. J Clin Neurosci. 2007; 14(11):1062-6. DOI: 10.1016/j.jocn.2006.11.008. View

15.

Zimmermann C, Winnefeld K, Streck S, Roskos M, Haberl R . Antioxidant status in acute stroke patients and patients at stroke risk. Eur Neurol. 2004; 51(3):157-61. DOI: 10.1159/000077662. View

16.

Michiels C, Raes M, Toussaint O, Remacle J . Importance of Se-glutathione peroxidase, catalase, and Cu/Zn-SOD for cell survival against oxidative stress. Free Radic Biol Med. 1994; 17(3):235-48. DOI: 10.1016/0891-5849(94)90079-5. View

17.

Kocaturk P, Akbostanci M, Isikay C, Ocal A, Tuncel D, Kavas G . Antioxidant status in cerebrovascular accident. Biol Trace Elem Res. 2001; 80(2):115-24. DOI: 10.1385/BTER:80:2:115. View

18.

Ferretti G, Bacchetti T, Masciangelo S, Nanetti L, Mazzanti L, Silvestrini M . Lipid peroxidation in stroke patients. Clin Chem Lab Med. 2007; 46(1):113-7. DOI: 10.1515/CCLM.2008.011. View

19.

Weigand M, Laipple A, Plaschke K, Eckstein H, Martin E, Bardenheuer H . Concentration changes of malondialdehyde across the cerebral vascular bed and shedding of L-selectin during carotid endarterectomy. Stroke. 1999; 30(2):306-11. DOI: 10.1161/01.str.30.2.306. View

20.

Sheikh N, Tavilani H, Rezaie A, Vaisi-Raygani A, Salimi S . Relationship between estradiol and antioxidant enzymes activity of ischemic stroke. J Biomed Biotechnol. 2009; 2009:841468. PMC: 2737135. DOI: 10.1155/2009/841468. View