AID Expression Increased by TNF-α is Associated with Class Switch Recombination of Igα Gene in Cancers

Overview

Journal Cell Mol Immunol

Date 2015 Apr 8

PMID 25849121

Citations 8

Authors

Zhi Duan

Hui Zheng

Haidan Liu

Ming Li

Min Tang

Xinxian Weng

Wei Yi

Ann M Bode

Ya Cao

Affiliations

Soon will be listed here.

Abstract

Recently, immunoglobulins (Igs) were unexpectedly found to be expressed in epithelial cancers. Immunoglobulin class switching or class switch recombination (CSR) is a natural biological process that alters a B cell's production of antibodies (immunoglobulins) from one class to another. However, the mechanism of CSR of Ig genes in cancer is still unknown. Here, we confirmed by detecting the hallmark of CSR that the Igα gene in cancer underwent CSR. Then we focused on activation-induced cytidine deaminase (AID), a crucial factor for initiating CSR. Further studies using tumor necrosis factor (TNF)-α stimulation and specific inhibitor of NF-κB revealed that TNF-α could increase AID expression through NF-κB signaling. Finally, we demonstrated that AID could co-localize with protein kinase A and bind to the switching (Sα) region of the Igα gene. Overexpression of AID obviously enhanced Igα heavy chain expression and its binding ability to the Sα region. These findings indicated that TNF-α-induced AID expression is involved with CSR in cancer.

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