Effects of Bifidobacterium Supplementation on Intestinal Microbiota Composition and the Immune Response in Healthy Infants

Overview

Journal World J Pediatr

Specialty Pediatrics

Date 2015 Apr 8

PMID 25846071

Citations 38

Authors

Bing-Bing Wu

Yi Yang

Xiu Xu

Wei-Ping Wang

Affiliations

Soon will be listed here.

Abstract

Background: Intestinal microbiotas are thought to be the most important source of maturational stimuli to the development of the immune system. However, few studies have focused on the development of T helper (Th) 1 immune response and antibody response to vaccinations in healthy infants, especially in a large cohort. Through this randomized, double-blind control trial, we investigated the effects of Bifidobacterium longum BB536 (BB536) supplementation on intestinal microbiota composition and the immune response in term infants.

Methods: In total, 300 healthy newborns were recruited, randomized and fed formula either supplemented with BB536 or with no supplementation. Stool samples were analyzed at months 2, 4 and 11. The representative cytokine for Th1 [interferon-γ (IFN-γ)] and Th2 [interleukin-4 (IL-4)] secretion cells were measured using enzyme-linked immunospot assay at 4 and 7 months of age. The antibody response to vaccines was measured at months 7 and 11.

Results: A total of 264 infants completed the study. The amount of bifidobacteria and the bifidobacteria/ Enterobacteriaceae ratio (B/E) were significantly higher in the BB536 supplementation group at months 2 and 4. The number of IFN-γ secretion cells and the ratio of IFN-γ/IL-4 secretion cells were increased in the BB536 supplementation group at 7 months. Moreover, the higher value of B/E in the early stages seems to be related to the increased Th1 response. No difference was observed between groups in the antibody response after vaccination.

Conclusion: BB536 has positive effects on establishing a healthy intestinal microbiota early in life, and it also plays an important role in improving the Th1 immune response.

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References

Rochat F, Serrant P, Aeschlimann J, Schiffrin E . Modulation of nonspecific mechanisms of defense by lactic acid bacteria: effective dose. J Dairy Sci. 1999; 82(5):863-9. DOI: 10.3168/jds.S0022-0302(99)75304-X. View

West C, Hernell O, Andersson Y, Sjostedt M, Hammarstrom M . Probiotic effects on T-cell maturation in infants during weaning. Clin Exp Allergy. 2012; 42(4):540-9. DOI: 10.1111/j.1365-2222.2011.03941.x. View

Macdonald T, Bell I . Probiotics and the immune response to vaccines. Proc Nutr Soc. 2010; 69(3):442-6. DOI: 10.1017/S0029665110001758. View

Kim J, Kwon J, Ahn S, Lee S, Han Y, Choi Y . Effect of probiotic mix (Bifidobacterium bifidum, Bifidobacterium lactis, Lactobacillus acidophilus) in the primary prevention of eczema: a double-blind, randomized, placebo-controlled trial. Pediatr Allergy Immunol. 2009; 21(2 Pt 2):e386-93. DOI: 10.1111/j.1399-3038.2009.00958.x. View

Bezirtzoglou E, Maipa V, Chotoura N, Apazidou E, Tsiotsias A, Voidarou C . Occurrence of Bifidobacterium in the intestine of newborns by fluorescence in situ hybridization. Comp Immunol Microbiol Infect Dis. 2006; 29(5-6):345-52. DOI: 10.1016/j.cimid.2006.09.002. View

Strachan D . Family size, infection and atopy: the first decade of the "hygiene hypothesis". Thorax. 2000; 55 Suppl 1:S2-10. PMC: 1765943. DOI: 10.1136/thorax.55.suppl_1.s2. View

Odamaki T, Xiao J, Iwabuchi N, Sakamoto M, Takahashi N, Kondo S . Influence of Bifidobacterium longum BB536 intake on faecal microbiota in individuals with Japanese cedar pollinosis during the pollen season. J Med Microbiol. 2007; 56(Pt 10):1301-1308. DOI: 10.1099/jmm.0.47306-0. View

Soh S, Ong D, Gerez I, Zhang X, Chollate P, Shek L . Effect of probiotic supplementation in the first 6 months of life on specific antibody responses to infant Hepatitis B vaccination. Vaccine. 2010; 28(14):2577-9. DOI: 10.1016/j.vaccine.2010.01.020. View

Miraglia Del Giudice M, Leonardi S, Maiello N, Brunese F . Food allergy and probiotics in childhood. J Clin Gastroenterol. 2010; 44 Suppl 1:S22-5. DOI: 10.1097/MCG.0b013e3181e102a7. View

10.

Borchers A, Selmi C, Meyers F, Keen C, Gershwin M . Probiotics and immunity. J Gastroenterol. 2009; 44(1):26-46. DOI: 10.1007/s00535-008-2296-0. View

11.

Dong P, Yang Y, Wang W . The role of intestinal bifidobacteria on immune system development in young rats. Early Hum Dev. 2010; 86(1):51-8. DOI: 10.1016/j.earlhumdev.2010.01.002. View

12.

Purchiaroni F, Tortora A, Gabrielli M, Bertucci F, Gigante G, Ianiro G . The role of intestinal microbiota and the immune system. Eur Rev Med Pharmacol Sci. 2013; 17(3):323-33. View

13.

Rupa P, Mine Y . Recent advances in the role of probiotics in human inflammation and gut health. J Agric Food Chem. 2012; 60(34):8249-56. DOI: 10.1021/jf301903t. View

14.

Mullie C, Yazourh A, Thibault H, Odou M, Singer E, Kalach N . Increased poliovirus-specific intestinal antibody response coincides with promotion of Bifidobacterium longum-infantis and Bifidobacterium breve in infants: a randomized, double-blind, placebo-controlled trial. Pediatr Res. 2004; 56(5):791-5. DOI: 10.1203/01.PDR.0000141955.47550.A0. View

15.

Sanders M . Probiotics: definition, sources, selection, and uses. Clin Infect Dis. 2008; 46 Suppl 2:S58-61. DOI: 10.1086/523341. View

16.

Namba K, Hatano M, Yaeshima T, Takase M, Suzuki K . Effects of Bifidobacterium longum BB536 administration on influenza infection, influenza vaccine antibody titer, and cell-mediated immunity in the elderly. Biosci Biotechnol Biochem. 2010; 74(5):939-45. DOI: 10.1271/bbb.90749. View

17.

Russell S, Gold M, Willing B, Thorson L, McNagny K, Finlay B . Perinatal antibiotic treatment affects murine microbiota, immune responses and allergic asthma. Gut Microbes. 2013; 4(2):158-64. PMC: 3595077. DOI: 10.4161/gmic.23567. View

18.

Ygberg S, Nilsson A . The developing immune system - from foetus to toddler. Acta Paediatr. 2011; 101(2):120-7. DOI: 10.1111/j.1651-2227.2011.02494.x. View

19.

Youngster I, Kozer E, Lazarovitch Z, Broide E, Goldman M . Probiotics and the immunological response to infant vaccinations: a prospective, placebo controlled pilot study. Arch Dis Child. 2011; 96(4):345-9. DOI: 10.1136/adc.2010.197459. View

20.

Savilahti E, Kukkonen K, Kuitunen M . Probiotics in the treatment and prevention of allergy in children. World Allergy Organ J. 2013; 2(5):69-76. PMC: 3651021. DOI: 10.1097/WOX.0b013e3181a45ee5. View