Virulence Characterization of Campylobacter Jejuni Isolated from Resident Wild Birds in Tokachi Area, Japan

Overview

Journal J Vet Med Sci

Specialty Veterinary Medicine

Date 2015 Apr 7

PMID 25843040

Citations 11

Authors

Anselme Shyaka

Akiko Kusumoto

Warangkhana Chaisowwong

Yoshiki Okouchi

Shinya Fukumoto

Aya Yoshimura

Keiko Kawamoto

Affiliations

Soon will be listed here.

Abstract

The prevalence of Campylobacter jejuni in wild birds is a potential hazard for human and animal health. The aim of this study was to establish the prevalence of C. jejuni in wild birds in Tokachi area, Hokkaido, Japan and investigate their virulence in vitro. In total, 173 cloacal swabs from individual wild birds were collected for the detection of Campylobacter spp. Thirty four samples (19.7%) were positive for Campylobacter of which 94.1% (32/34 samples) were C. jejuni. Additionally, one C. coli and one C. fetus were isolated. Seven C. jejuni isolates (one from crows and the other from pigeons) had important virulence genes including all three CDT genes (cdtA, cdtB and cdtC) and flaA, flaB, ciaB and cadF, and the other isolates were lacking cdtA gene. Further studies on in vitro virulence-associated phenotypes, such as motility assay on soft agar and invasion assay in Caco-2 cells, were performed. The wild bird C. jejuni isolates adhered and invaded human cells. Although the numbers of viable intracellular bacteria of wild bird isolates were lower than a type strain NCTC11168, they persisted at 48-hr and underwent replication in host cells.

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References

Poly F, Ewing C, Goon S, Hickey T, Rockabrand D, Majam G . Heterogeneity of a Campylobacter jejuni protein that is secreted through the flagellar filament. Infect Immun. 2007; 75(8):3859-67. PMC: 1951984. DOI: 10.1128/IAI.00159-07. View

Fukuyama M, Kamimura T, Itoh T, Saito K, Takahashi M, Sakai S . Distribution of Campylobacter jejuni in wild birds and serogroup of isolates by slide agglutination technique. Nihon Juigaku Zasshi. 1986; 48(3):487-93. DOI: 10.1292/jvms1939.48.487. View

French N, Midwinter A, Holland B, Collins-Emerson J, Pattison R, Colles F . Molecular epidemiology of Campylobacter jejuni isolates from wild-bird fecal material in children's playgrounds. Appl Environ Microbiol. 2008; 75(3):779-83. PMC: 2632120. DOI: 10.1128/AEM.01979-08. View

Chaisowwong W, Kusumoto A, Hashimoto M, Harada T, Maklon K, Kawamoto K . Physiological characterization of Campylobacter jejuni under cold stresses conditions: its potential for public threat. J Vet Med Sci. 2011; 74(1):43-50. DOI: 10.1292/jvms.11-0305. View

Mishu B, Blaser M . Role of infection due to Campylobacter jejuni in the initiation of Guillain-Barré syndrome. Clin Infect Dis. 1993; 17(1):104-8. DOI: 10.1093/clinids/17.1.104. View

Sprenger H, Zechner E, Gorkiewicz G . So close and yet so far - Molecular Microbiology of Campylobacter fetus subspecies. Eur J Microbiol Immunol (Bp). 2014; 2(1):66-75. PMC: 3933992. DOI: 10.1556/EuJMI.2.2012.1.10. View

Kinjo T, Morishige M, Minamoto N, Fukushi H . Prevalence of Campylobacter jejuni in feral pigeons. Nihon Juigaku Zasshi. 1983; 45(6):833-5. DOI: 10.1292/jvms1939.45.833. View

Beery J, Hugdahl M, Doyle M . Colonization of gastrointestinal tracts of chicks by Campylobacter jejuni. Appl Environ Microbiol. 1988; 54(10):2365-70. PMC: 204261. DOI: 10.1128/aem.54.10.2365-2370.1988. View

Biswas D, Itoh K, Sasakawa C . Uptake pathways of clinical and healthy animal isolates of Campylobacter jejuni into INT-407 cells. FEMS Immunol Med Microbiol. 2000; 29(3):203-11. DOI: 10.1111/j.1574-695X.2000.tb01524.x. View

10.

Hughes L, Bennett M, Coffey P, Elliott J, Jones T, Jones R . Molecular epidemiology and characterization of Campylobacter spp. isolated from wild bird populations in northern England. Appl Environ Microbiol. 2009; 75(10):3007-15. PMC: 2681652. DOI: 10.1128/AEM.02458-08. View

11.

Dasti J, Tareen A, Lugert R, Zautner A, Gross U . Campylobacter jejuni: a brief overview on pathogenicity-associated factors and disease-mediating mechanisms. Int J Med Microbiol. 2009; 300(4):205-11. DOI: 10.1016/j.ijmm.2009.07.002. View

12.

Scott A, Timms A, Connerton P, Loc Carrillo C, Adzfa Radzum K, Connerton I . Genome dynamics of Campylobacter jejuni in response to bacteriophage predation. PLoS Pathog. 2007; 3(8):e119. PMC: 1950947. DOI: 10.1371/journal.ppat.0030119. View

13.

Weis A, Miller W, Byrne B, Chouicha N, Boyce W, Townsend A . Prevalence and pathogenic potential of campylobacter isolates from free-living, human-commensal american crows. Appl Environ Microbiol. 2013; 80(5):1639-44. PMC: 3957608. DOI: 10.1128/AEM.03393-13. View

14.

Ichiyama S, Hirai S, Minami T, Nishiyama Y, Shimizu S, Shimokata K . Campylobacter fetus subspecies fetus cellulitis associated with bacteremia in debilitated hosts. Clin Infect Dis. 1998; 27(2):252-5. DOI: 10.1086/514654. View

15.

Allos B . Campylobacter jejuni Infections: update on emerging issues and trends. Clin Infect Dis. 2001; 32(8):1201-6. DOI: 10.1086/319760. View

16.

Chuma T, Hashimoto S, Okamoto K . Detection of thermophilic Campylobacter from sparrows by multiplex PCR: the role of sparrows as a source of contamination of broilers with Campylobacter. J Vet Med Sci. 2001; 62(12):1291-5. DOI: 10.1292/jvms.62.1291. View

17.

Black R, Levine M, Clements M, Hughes T, Blaser M . Experimental Campylobacter jejuni infection in humans. J Infect Dis. 1988; 157(3):472-9. DOI: 10.1093/infdis/157.3.472. View

18.

De Melo M, Gabbiani G, Pechere J . Cellular events and intracellular survival of Campylobacter jejuni during infection of HEp-2 cells. Infect Immun. 1989; 57(7):2214-22. PMC: 313863. DOI: 10.1128/iai.57.7.2214-2222.1989. View

19.

SHANE S . The significance of Campylobacter jejuni infection in poultry: a review. Avian Pathol. 1992; 21(2):189-213. DOI: 10.1080/03079459208418836. View

20.

Matsusaki S, Katayama A, Itagaki K, Yamagata H, Tanaka K, Yamami T . Prevalence of Campylobacter jejuni and Campylobacter coli among wild and domestic animals in Yamaguchi Prefecture. Microbiol Immunol. 1986; 30(12):1317-22. DOI: 10.1111/j.1348-0421.1986.tb03052.x. View