Parameters and Characteristics Governing Cellular Internalization and Trans-barrier Trafficking of Nanostructures

Overview

Journal Int J Nanomedicine

Publisher Dove Medical Press

Specialty Biotechnology

Date 2015 Apr 3

PMID 25834433

Citations 73

Authors

Karmani Murugan

Yahya E Choonara

Pradeep Kumar

Divya Bijukumar

Lisa C du Toit

Viness Pillay

Affiliations

Soon will be listed here.

Abstract

Cellular internalization and trans-barrier transport of nanoparticles can be manipulated on the basis of the physicochemical and mechanical characteristics of nanoparticles. Research has shown that these factors significantly influence the uptake of nanoparticles. Dictating these characteristics allows for the control of the rate and extent of cellular uptake, as well as delivering the drug-loaded nanosystem intra-cellularly, which is imperative for drugs that require a specific cellular level to exert their effects. Additionally, physicochemical characteristics of the nanoparticles should be optimal for the nanosystem to bypass the natural restricting phenomena of the body and act therapeutically at the targeted site. The factors at the focal point of emerging smart nanomedicines include nanoparticle size, surface charge, shape, hydrophobicity, surface chemistry, and even protein and ligand conjugates. Hence, this review discusses the mechanism of internalization of nanoparticles and ideal nanoparticle characteristics that allow them to evade the biological barriers in order to achieve optimal cellular uptake in different organ systems. Identifying these parameters assists with the progression of nanomedicine as an outstanding vector of pharmaceuticals.

Citing Articles

Nose-to-Brain Delivery of Biomimetic Nanoparticles for Glioblastoma Targeted Therapy.

Ferreira N, Leite C, Moreno N, Miranda R, Pincela Lins P, Rodero C ACS Appl Mater Interfaces. 2024; 17(1):484-499.

PMID: 39692595 PMC: 11783514. DOI: 10.1021/acsami.4c16837.

Recent Advances in the Development and Utilization of Nanoparticles for the Management of Malignant Solid Tumors.

Chavan D, Bhosale R, Thorat V, Shete A, Patil S, Tiwari D Cureus. 2024; 16(9):e70312.

PMID: 39469411 PMC: 11513206. DOI: 10.7759/cureus.70312.

Green synthesized silver nanoparticles, a sustainable approach for fruit and vegetable preservation: An overview.

Lieu M, Dang T, Nguyen T Food Chem X. 2024; 23:101664.

PMID: 39148528 PMC: 11324848. DOI: 10.1016/j.fochx.2024.101664.

Near-Infrared Emissive Super Penetrating Conjugated Polymer Dots for Intratumoral Imaging in 3D Tumor Spheroid Models.

Karabacak S, Coban B, Yildiz A, Yildiz U Adv Sci (Weinh). 2024; 11(35):e2403398.

PMID: 39023182 PMC: 11425279. DOI: 10.1002/advs.202403398.

Unveiling Nanoparticles: Recent Approaches in Studying the Internalization Pattern of Iron Oxide Nanoparticles in Mono- and Multicellular Biological Structures.

Petcov T, Straticiuc M, Iancu D, Mirea D, Trusca R, Mereuta P J Funct Biomater. 2024; 15(6).

PMID: 38921542 PMC: 11204647. DOI: 10.3390/jfb15060169.

References

Geng Y, Dalhaimer P, Cai S, Tsai R, Tewari M, Minko T . Shape effects of filaments versus spherical particles in flow and drug delivery. Nat Nanotechnol. 2008; 2(4):249-55. PMC: 2740330. DOI: 10.1038/nnano.2007.70. View

Pelkmans L, Puntener D, Helenius A . Local actin polymerization and dynamin recruitment in SV40-induced internalization of caveolae. Science. 2002; 296(5567):535-9. DOI: 10.1126/science.1069784. View

Vllasaliu D, Fowler R, Garnett M, Eaton M, Stolnik S . Barrier characteristics of epithelial cultures modelling the airway and intestinal mucosa: a comparison. Biochem Biophys Res Commun. 2011; 415(4):579-85. DOI: 10.1016/j.bbrc.2011.10.108. View

Das M, Patil S, Bhargava N, Kang J, Riedel L, Seal S . Auto-catalytic ceria nanoparticles offer neuroprotection to adult rat spinal cord neurons. Biomaterials. 2007; 28(10):1918-25. PMC: 1913191. DOI: 10.1016/j.biomaterials.2006.11.036. View

Jallouli Y, Paillard A, Chang J, Sevin E, Betbeder D . Influence of surface charge and inner composition of porous nanoparticles to cross blood-brain barrier in vitro. Int J Pharm. 2007; 344(1-2):103-9. DOI: 10.1016/j.ijpharm.2007.06.023. View

Kumari S, Mg S, Mayor S . Endocytosis unplugged: multiple ways to enter the cell. Cell Res. 2010; 20(3):256-75. PMC: 7091825. DOI: 10.1038/cr.2010.19. View

Alonso M . Nanomedicines for overcoming biological barriers. Biomed Pharmacother. 2004; 58(3):168-72. DOI: 10.1016/j.biopha.2004.01.007. View

Ferrati S, McConnell K, Mack A, Sirisaengtaksin N, Diaz R, Bean A . Cellular communication via nanoparticle-transporting biovesicles. Nanomedicine (Lond). 2013; 9(5):581-592. PMC: 3947494. DOI: 10.2217/nnm.13.57. View

Liu Y, Shipton M, Ryan J, Kaufman E, Franzen S, Feldheim D . Synthesis, stability, and cellular internalization of gold nanoparticles containing mixed peptide-poly(ethylene glycol) monolayers. Anal Chem. 2007; 79(6):2221-9. DOI: 10.1021/ac061578f. View

10.

Wang Z, Tiruppathi C, Minshall R, Malik A . Size and dynamics of caveolae studied using nanoparticles in living endothelial cells. ACS Nano. 2009; 3(12):4110-6. PMC: 3643811. DOI: 10.1021/nn9012274. View

11.

Gratton S, Napier M, Ropp P, Tian S, DeSimone J . Microfabricated particles for engineered drug therapies: elucidation into the mechanisms of cellular internalization of PRINT particles. Pharm Res. 2008; 25(12):2845-52. PMC: 2593117. DOI: 10.1007/s11095-008-9654-8. View

12.

Lin A, Liu Y, Huang Y, Sun J, Wu Z, Zhang X . Glycyrrhizin surface-modified chitosan nanoparticles for hepatocyte-targeted delivery. Int J Pharm. 2008; 359(1-2):247-53. DOI: 10.1016/j.ijpharm.2008.03.039. View

13.

Mistry A, Stolnik S, Illum L . Nanoparticles for direct nose-to-brain delivery of drugs. Int J Pharm. 2009; 379(1):146-57. DOI: 10.1016/j.ijpharm.2009.06.019. View

14.

Pujals S, Giralt E . Proline-rich, amphipathic cell-penetrating peptides. Adv Drug Deliv Rev. 2008; 60(4-5):473-84. DOI: 10.1016/j.addr.2007.09.012. View

15.

Mousavi S, Malerod L, Berg T, Kjeken R . Clathrin-dependent endocytosis. Biochem J. 2003; 377(Pt 1):1-16. PMC: 1223844. DOI: 10.1042/BJ20031000. View

16.

Gratton S, Ropp P, Pohlhaus P, Luft J, Madden V, Napier M . The effect of particle design on cellular internalization pathways. Proc Natl Acad Sci U S A. 2008; 105(33):11613-8. PMC: 2575324. DOI: 10.1073/pnas.0801763105. View

17.

Park H, Cox D . Cdc42 regulates Fc gamma receptor-mediated phagocytosis through the activation and phosphorylation of Wiskott-Aldrich syndrome protein (WASP) and neural-WASP. Mol Biol Cell. 2009; 20(21):4500-8. PMC: 2770938. DOI: 10.1091/mbc.e09-03-0230. View

18.

Chithrani B, Ghazani A, Chan W . Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells. Nano Lett. 2006; 6(4):662-8. DOI: 10.1021/nl052396o. View

19.

Champion J, Katare Y, Mitragotri S . Making polymeric micro- and nanoparticles of complex shapes. Proc Natl Acad Sci U S A. 2007; 104(29):11901-4. PMC: 1924596. DOI: 10.1073/pnas.0705326104. View

20.

Bertrand N, Leroux J . The journey of a drug-carrier in the body: an anatomo-physiological perspective. J Control Release. 2011; 161(2):152-63. DOI: 10.1016/j.jconrel.2011.09.098. View