Affinity Selection of Epitope-based Vaccines Using a Bacteriophage Virus-like Particle Platform

Overview

Journal Curr Opin Virol

Publisher Elsevier

Specialty Microbiology

Date 2015 Apr 2

PMID 25829254

Citations 17

Authors

John P ORourke

David S Peabody

Bryce Chackerian

Affiliations

Soon will be listed here.

Abstract

Display of epitopes on virus-like particles (VLPs) is a highly effective technique for enhancing the immunogenicity of antigens that are poorly immunogenic in their native context. VLP-based vaccines can be used to elicit long-lasting, high-titer antibody responses against diverse target antigens, even self-antigens. Most VLP platform-based vaccines are rationally engineered; specific target epitopes or domains are arrayed so that they are displayed at high-valency on the surface of VLPs. In this review, we describe an alternate technique for vaccine discovery using VLPs. This strategy, analogous to filamentous phage display, allows bacteriophage VLP-based vaccines to be identified from a vast library of potential vaccines by affinity selection. This technology integrates epitope discovery and immunization functions into a single platform.

Citing Articles

The RNA Landscape of In Vivo-Assembled MS2 Virus-Like Particles as mRNA Carriers Reveals RNA Contamination from Host Viruses.

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One-plasmid double-expression system for preparation of MS2 virus-like particles packaging SARS-CoV-2 RNA.

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Virus-like Particles: Fundamentals and Biomedical Applications.

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