A Proteomic Analysis on Human Sperm Tail: Comparison Between Normozoospermia and Asthenozoospermia

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2015 Apr 1

PMID 25825237

Citations 34

Authors

Mahmoud Hashemitabar

Susan Sabbagh

Mahmoud Orazizadeh

Atta Ghadiri

Maryam Bahmanzadeh

Affiliations

Soon will be listed here.

Abstract

Purpose: Asthenozoospermia is a common cause of human male infertility characterized by reduced sperm motility. The molecular mechanism that impairs sperm motility is not fully understood. This study proposed to identify novel biomarkers by focusing on sperm tail proteomic analysis of asthenozoospermic patients.

Methods: Sperm were isolated from normozoospermic and asthenozoospermic semen samples. Tail fractions were obtained by sonication followed by Percoll gradient. The proteins were extracted by solubilization and subjected to two-dimensional gel electrophoresis (2-DE); then, the spots were analyzed using Image Master 2D Platinum software. The significantly increased/decreased amounts of proteins in the two groups were exploited by matrix-assisted laser desorption-ionization time-of-flight/time-of-flight (MALDI-TOF-TOF) mass spectrometry.

Results: Three hundred ninety protein spots were detected in both groups. Twenty-one protein spots that had significantly altered amounts (p < 0.05) were excised and exploited using MALDI-TOF-TOF mass spectrometry. They led to the identification of the following 14 unique proteins: Tubulin beta 2B; glutathione S-transferase Mu 3; keratin, type II cytoskeletal 1; outer dense fiber protein 2; voltage-dependent anion-selective channel protein 2; A-kinase anchor protein 4; cytochrome c oxidase subunit 6B; sperm protein associated with the nucleus on the X chromosome B; phospholipid hydroperoxide glutathione peroxidase-mitochondrial; isoaspartyl peptidase/L-asparaginase; heat shock-related 70 kDa protein 2; stress-70 protein, mitochondrial; glyceraldehyde-3-phosphate dehydrogenase, testis-specific and clusterin.

Conclusion: Fourteen proteins present in different amounts in asthenozoospermic sperm tail samples were identified, four of which are reported here for the first time. These proteins might be used as markers for the better diagnosis of sperm dysfunctions, targets for male contraceptive development, and to predict embryo quality.

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References

Schneider H, Berthold J, Bauer M, Dietmeier K, Guiard B, Brunner M . Mitochondrial Hsp70/MIM44 complex facilitates protein import. Nature. 1994; 371(6500):768-74. DOI: 10.1038/371768a0. View

Francavilla S, Pelliccione F, Cordeschi G, Necozione S, Santucci R, Bocchio M . Utrastructural analysis of asthenozoospermic ejaculates in the era of assisted procreation. Fertil Steril. 2006; 85(4):940-6. DOI: 10.1016/j.fertnstert.2005.10.027. View

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

Kwon W, Park Y, Mohamed E, Pang M . Voltage-dependent anion channels are a key factor of male fertility. Fertil Steril. 2012; 99(2):354-61. DOI: 10.1016/j.fertnstert.2012.09.021. View

Peknicova J, Pexidrova M, Kubatova A, Koubek P, Tepla O, Sulimenko T . Expression of beta-tubulin epitope in human sperm with pathological spermiogram. Fertil Steril. 2007; 88(4 Suppl):1120-8. DOI: 10.1016/j.fertnstert.2006.12.070. View

Liu B, Wang P, Wang Z, Jia Y, Niu X, Wang W . Analysis and difference of voltage-dependent anion channel mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia. J Assist Reprod Genet. 2010; 27(12):719-24. PMC: 2997945. DOI: 10.1007/s10815-010-9466-8. View

Mosher W, PRATT W . Fecundity and infertility in the United States: incidence and trends. Fertil Steril. 1991; 56(2):192-3. View

Ibrahim N, Gilbert G, Loseth K, Crabo B . Correlation between clusterin-positive spermatozoa determined by flow cytometry in bull semen and fertility. J Androl. 2000; 21(6):887-94. View

Cao W, Gerton G, Moss S . Proteomic profiling of accessory structures from the mouse sperm flagellum. Mol Cell Proteomics. 2006; 5(5):801-10. DOI: 10.1074/mcp.M500322-MCP200. View

10.

Chan C, Shui H, Wu C, Wang C, Sun G, Chen H . Motility and protein phosphorylation in healthy and asthenozoospermic sperm. J Proteome Res. 2009; 8(11):5382-6. DOI: 10.1021/pr9003932. View

11.

Suryawanshi A, Khan S, Gajbhiye R, Gurav M, Khole V . Differential proteomics leads to identification of domain-specific epididymal sperm proteins. J Androl. 2010; 32(3):240-59. DOI: 10.2164/jandrol.110.010967. View

12.

Zhao C, Huo R, Wang F, Lin M, Zhou Z, Sha J . Identification of several proteins involved in regulation of sperm motility by proteomic analysis. Fertil Steril. 2006; 87(2):436-8. DOI: 10.1016/j.fertnstert.2006.06.057. View

13.

Baker M, Naumovski N, Hetherington L, Weinberg A, Velkov T, Aitken R . Head and flagella subcompartmental proteomic analysis of human spermatozoa. Proteomics. 2012; 13(1):61-74. DOI: 10.1002/pmic.201200350. View

14.

Eddy E, Toshimori K, OBrien D . Fibrous sheath of mammalian spermatozoa. Microsc Res Tech. 2003; 61(1):103-15. DOI: 10.1002/jemt.10320. View

15.

Li Y, He W, Mandal A, Kim Y, Digilio L, Klotz K . CABYR binds to AKAP3 and Ropporin in the human sperm fibrous sheath. Asian J Androl. 2011; 13(2):266-74. PMC: 3739192. DOI: 10.1038/aja.2010.149. View

16.

Bush L, Herr J, Wolkowicz M, Sherman N, Shore A, Flickinger C . A novel asparaginase-like protein is a sperm autoantigen in rats. Mol Reprod Dev. 2002; 62(2):233-47. DOI: 10.1002/mrd.10092. View

17.

Miki K, Qu W, Goulding E, Willis W, Bunch D, Strader L . Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme, is required for sperm motility and male fertility. Proc Natl Acad Sci U S A. 2004; 101(47):16501-6. PMC: 534542. DOI: 10.1073/pnas.0407708101. View

18.

Moore F . Male sperm motility dictated by mother's mtDNA. Am J Hum Genet. 2000; 67(3):543-8. PMC: 1287514. DOI: 10.1086/303061. View

19.

Ursini F, Heim S, Kiess M, Maiorino M, Roveri A, Wissing J . Dual function of the selenoprotein PHGPx during sperm maturation. Science. 1999; 285(5432):1393-6. DOI: 10.1126/science.285.5432.1393. View

20.

Miki K, Willis W, Brown P, Goulding E, Fulcher K, Eddy E . Targeted disruption of the Akap4 gene causes defects in sperm flagellum and motility. Dev Biol. 2002; 248(2):331-42. DOI: 10.1006/dbio.2002.0728. View