Lpg0393 of Legionella Pneumophila is a Guanine-nucleotide Exchange Factor for Rab5, Rab21 and Rab22

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2015 Mar 31

PMID 25821953

Citations 9

Authors

Young-Sik Sohn

Ho-Chul Shin

Wei Sun Park

Jianning Ge

Chan-Hee Kim

Bok Luel Lee

Won Do Heo

Jae U Jung

Daniel John Rigden

Byung-Ha Oh

Affiliations

Soon will be listed here.

Abstract

Legionella pneumophila, a human intracellular pathogen, encodes about 290 effector proteins that are translocated into host cells through a secretion machinery. Some of these proteins have been shown to manipulate or subvert cellular processes during infection, but functional roles of a majority of them remain unknown. Lpg0393 is a newly identified Legionella effector classified as a hypothetical protein. Through X-ray crystallographic analysis, we show that Lpg0393 contains a Vps9-like domain, which is structurally most similar to the catalytic core of human Rabex-5 that activates the endosomal Rab proteins Rab5, Rab21 and Rab22. Consistently, Lpg0393 exhibited a guanine-nucleotide exchange factor activity toward the endosomal Rabs. This work identifies the first example of a bacterial guanine-nucleotide exchange factor that is active towards the Rab5 sub-cluster members, implying that the activation of these Rab proteins might be advantageous for the intracellular survival of Legionella.

Citing Articles

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