Expression, Sorting, and Segregation of Golgi Proteins During Germ Cell Differentiation in the Testis

Overview

Journal Mol Biol Cell

Specialties Cell Biology
Molecular Biology

Date 2015 Mar 27

PMID 25808494

Citations 12

Authors

Catherine E Au

Louis Hermo

Elliot Byrne

Jeffrey Smirle

Ali Fazel

Paul H G Simon

Robert E Kearney

Pamela H Cameron

Charles E Smith

Hojatollah Vali

Julia Fernandez-Rodriguez

Kewei Ma

Tommy Nilsson

John J M Bergeron

Affiliations

Soon will be listed here.

Abstract

The molecular basis of changes in structure, cellular location, and function of the Golgi apparatus during male germ cell differentiation is unknown. To deduce cognate Golgi proteins, we isolated germ cell Golgi fractions, and 1318 proteins were characterized, with 20 localized in situ. The most abundant protein, GL54D of unknown function, is characterized as a germ cell-specific Golgi-localized type II integral membrane glycoprotein. TM9SF3, also of unknown function, was revealed to be a universal Golgi marker for both somatic and germ cells. During acrosome formation, several Golgi proteins (GBF1, GPP34, GRASP55) localize to both the acrosome and Golgi, while GL54D, TM9SF3, and the Golgi trafficking protein TMED7/p27 are segregated from the acrosome. After acrosome formation, GL54D, TM9SF3, TMED4/p25, and TMED7/p27 continue to mark Golgi identity as it migrates away from the acrosome, while the others (GBF1, GPP34, GRASP55) remain in the acrosome and are progressively lost in later steps of differentiation. Cytoplasmic HSP70.2 and the endoplasmic reticulum luminal protein-folding enzyme PDILT are also Golgi recruited but only during acrosome formation. This resource identifies abundant Golgi proteins that are expressed differentially during mitosis, meiosis, and postacrosome Golgi migration, including the last step of differentiation.

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