Andrographolide Enhances Proliferation and Prevents Dedifferentiation of Rabbit Articular Chondrocytes: an in Vitro Study

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2015 Mar 25

PMID 25802548

Citations 4

Authors

Li-Ke Luo

Qing-Jun Wei

Lei Liu

Li Zheng

Jin-Min Zhao

Affiliations

Soon will be listed here.

Abstract

As the main active constituent of Andrographis paniculata that was applied in treatment of many diseases including inflammation in ancient China, andrographolide (ANDRO) was found to facilitate reduction of edema and analgesia in arthritis. This suggested that ANDRO may be promising anti-inflammatory agent to relieve destruction and degeneration of cartilage after inflammation. In this study, the effect of ANDRO on rabbit articular chondrocytes in vitro was investigated. Results showed that not more than 8 μM ANDRO did no harm to chondrocytes (P < 0.05). DNA content and glycosaminoglycan (GAG) /DNA were, respectively, improved in ANDRO groups comparing to the control (P < 0.05). ANDRO could promote expression of aggrecan, collagen II, and Sox9 genes while downregulating expression of collagen I gene (P < 0.05). Furthermore, hypertrophy that may result in chondrocyte ossification could not be detected in all groups (P > 0.05). The viability assay, hematoxylin-eosin, safranin O, and immunohistochemical staining also showed better performances in ANDRO groups. As to the doses, 3 μM ANDRO showed the best performance. The results indicate that ANDRO can accelerate proliferation of rabbit articular chondrocytes in vitro and meanwhile maintain the phenotype, which may provide valuable references for further exploration on arthritis.

Citing Articles

Corrigendum to "Andrographolide Enhances Proliferation and Prevents Dedifferentiation of Rabbit Articular Chondrocytes: An Study".

Luo L, Wei Q, Liu L, Zheng L, Zhao J Evid Based Complement Alternat Med. 2022; 2022:9759547.

PMID: 36467558 PMC: 9718620. DOI: 10.1155/2022/9759547.

Expression of Concern on "Andrographolide Enhances Proliferation and Prevents Dedifferentiation of Rabbit Articular Chondrocytes: An In Vitro Study".

And Alternative Medicine E Evid Based Complement Alternat Med. 2021; 2021:4752718.

PMID: 33564321 PMC: 7850830. DOI: 10.1155/2021/4752718.

Dedifferentiation: inspiration for devising engineering strategies for regenerative medicine.

Yao Y, Wang C NPJ Regen Med. 2020; 5:14.

PMID: 32821434 PMC: 7395755. DOI: 10.1038/s41536-020-00099-8.

[Andrographolide-releasing collagen scaffold enhance the ability of chondrocytes to maintain their specific phenotype under inflammatory environment ].

Xu L, Liu H, Dai Y, Li Y, Chen W Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2018; 35(6):905-913.

PMID: 30583316 PMC: 9935195. DOI: 10.7507/1001-5515.201804025.

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