Proteins Transported in Slow Components a and B of Axonal Transport Are Distributed Differently in the Transverse Plane of the Axon

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1985 Apr 1

PMID 2579956

Citations 7

Authors

K Heriot

P Gambetti

R J Lasek

Affiliations

Soon will be listed here.

Abstract

The distribution of the proteins migrating with the slow components a (SCa) and b (SCb) of axonal transport were studied in cross-sections of axons with electron microscope autoradiography. Radiolabeled amino acids were injected into the hypoglossal nucleus of rabbits and after 15 d, the animals were killed. Hypoglossal nerves were processed either for SDS-polyacrylamide gel electrophoresis fluorography to identify and locate the two components of slow transport, or for quantitative electron microscope autoradiography. Proteins transported in SCa were found to be uniformly distributed within the cross-section of the axon. Labeled SCb proteins were also found throughout the axonal cross-section, but the subaxolemmal region of the axon contained 2.5 times more SCb radioactivity than any comparable area in the remainder of the axon.

Citing Articles

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Cytoskeletal requirements in axonal transport of slow component-b.

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Functional analysis of dynactin and cytoplasmic dynein in slow axonal transport.

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Rapidly transported organelles containing membrane and cytoskeletal components: their relation to axonal growth.

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Two classes of actin microfilaments are associated with the inner cytoskeleton of axons.

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