Electrical and Electrochemical Monitoring of Nucleic Acid Amplification

Overview

Journal Front Bioeng Biotechnol

Date 2015 Mar 24

PMID 25798440

Citations 6

Authors

Tatsuro Goda

Miyuki Tabata

Yuji Miyahara

Affiliations

Soon will be listed here.

Abstract

Nucleic acid amplification is a gold standard technique for analyzing a tiny amount of nucleotides in molecular biology, clinical diagnostics, food safety, and environmental testing. Electrical and electrochemical monitoring of the amplification process draws attention over conventional optical methods because of the amenability toward point-of-care applications as there is a growing demand for nucleic acid sensing in situations outside the laboratory. A number of electrical and electrochemical techniques coupled with various amplification methods including isothermal amplification have been reported in the last 10 years. In this review, we highlight recent developments in the electrical and electrochemical monitoring of nucleic acid amplification.

Citing Articles

Cascaded signal amplification strategy for ultra-specific, ultra-sensitive, and visual detection of Shigella flexneri.

Shi Y, Tan Q, Gong T, Li Q, Zhu Y, Duan X Mikrochim Acta. 2024; 191(5):271.

PMID: 38632191 DOI: 10.1007/s00604-024-06309-0.

Lateral Flow Assay for Hepatitis B Detection: A Review of Current and New Assays.

Abu N, Bakhori N, Shueb R Micromachines (Basel). 2023; 14(6).

PMID: 37374824 PMC: 10301844. DOI: 10.3390/mi14061239.

Detection of YAP1 and AR-V7 mRNA for Prostate Cancer Prognosis Using an ISFET Lab-On-Chip Platform.

Broomfield J, Kalofonou M, Pataillot-Meakin T, Powell S, Fernandes R, Moser N ACS Sens. 2022; 7(11):3389-3398.

PMID: 36368032 PMC: 9706784. DOI: 10.1021/acssensors.2c01463.

Bridging the gap between development of point-of-care nucleic acid testing and patient care for sexually transmitted infections.

Hsieh K, Melendez J, Gaydos C, Wang T Lab Chip. 2022; 22(3):476-511.

PMID: 35048928 PMC: 9035340. DOI: 10.1039/d1lc00665g.

Electrochemical Biosensors Combined with Isothermal Amplification for Quantitative Detection of Nucleic Acids.

Tabata M, Yao B, Seichi A, Suzuki K, Miyahara Y Methods Mol Biol. 2017; 1572:135-151.

PMID: 28299686 PMC: 7122880. DOI: 10.1007/978-1-4939-6911-1_10.

References

Lizardi P, Huang X, Zhu Z, Thomas D, Ward D . Mutation detection and single-molecule counting using isothermal rolling-circle amplification. Nat Genet. 1998; 19(3):225-32. DOI: 10.1038/898. View

Chen Y, Xu J, Su J, Xiang Y, Yuan R, Chai Y . In situ hybridization chain reaction amplification for universal and highly sensitive electrochemiluminescent detection of DNA. Anal Chem. 2012; 84(18):7750-5. DOI: 10.1021/ac3012285. View

Ji H, Yan F, Lei J, Ju H . Ultrasensitive electrochemical detection of nucleic acids by template enhanced hybridization followed with rolling circle amplification. Anal Chem. 2012; 84(16):7166-71. DOI: 10.1021/ac3015356. View

Del Giallo M, Lucarelli F, Cosulich E, Pistarino E, Santamaria B, Marrazza G . Steric factors controlling the surface hybridization of PCR amplified sequences. Anal Chem. 2005; 77(19):6324-30. DOI: 10.1021/ac0506175. View

Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N . Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000; 28(12):E63. PMC: 102748. DOI: 10.1093/nar/28.12.e63. View

Zhou L, Ou L, Chu X, Shen G, Yu R . Aptamer-based rolling circle amplification: a platform for electrochemical detection of protein. Anal Chem. 2007; 79(19):7492-500. DOI: 10.1021/ac071059s. View

Fang T, Ramalingam N, Xian-Dui D, Ngin T, Xianting Z, Lai Kuan A . Real-time PCR microfluidic devices with concurrent electrochemical detection. Biosens Bioelectron. 2009; 24(7):2131-6. DOI: 10.1016/j.bios.2008.11.009. View

Defever T, Druet M, Evrard D, Marchal D, Limoges B . Real-time electrochemical PCR with a DNA intercalating redox probe. Anal Chem. 2011; 83(5):1815-21. DOI: 10.1021/ac1033374. View

Ronaghi M, Uhlen M, Nyren P . A sequencing method based on real-time pyrophosphate. Science. 1998; 281(5375):363, 365. DOI: 10.1126/science.281.5375.363. View

10.

Cheng W, Ding S, Li Q, Yu T, Yin Y, Ju H . A simple electrochemical aptasensor for ultrasensitive protein detection using cyclic target-induced primer extension. Biosens Bioelectron. 2012; 36(1):12-7. DOI: 10.1016/j.bios.2012.03.032. View

11.

Lee H, Kim B, Kim K, Kim Y, Kim J, Oh M . A sensitive method to detect Escherichia coli based on immunomagnetic separation and real-time PCR amplification of aptamers. Biosens Bioelectron. 2009; 24(12):3550-5. DOI: 10.1016/j.bios.2009.05.010. View

12.

Vincent M, Xu Y, Kong H . Helicase-dependent isothermal DNA amplification. EMBO Rep. 2004; 5(8):795-800. PMC: 1249482. DOI: 10.1038/sj.embor.7400200. View

13.

Murakami T, Sumaoka J, Komiyama M . Sensitive isothermal detection of nucleic-acid sequence by primer generation-rolling circle amplification. Nucleic Acids Res. 2008; 37(3):e19. PMC: 2647323. DOI: 10.1093/nar/gkn1014. View

14.

Yan L, Zhou J, Zheng Y, Gamson A, Roembke B, Nakayama S . Isothermal amplified detection of DNA and RNA. Mol Biosyst. 2014; 10(5):970-1003. DOI: 10.1039/c3mb70304e. View

15.

Pioch D, Schweder T, Jurgen B . Novel developments for improved detection of specific mRNAs by DNA chips. Appl Microbiol Biotechnol. 2008; 80(6):953-63. DOI: 10.1007/s00253-008-1680-2. View

16.

Anbu S, Kamalraj S, Paul A, Jayabaskaran C, Pombeiro A . The phenanthroimidazole-based dizinc(II) complex as a fluorescent probe for the pyrophosphate ion as generated in polymerase chain reactions and pyrosequencing. Dalton Trans. 2015; 44(9):3930-3. DOI: 10.1039/c4dt03590a. View

17.

Fu L, Tang D, Zhuang J, Lai W, Que X, Chen G . Hybridization-induced isothermal cycling signal amplification for sensitive electronic detection of nucleic acid. Biosens Bioelectron. 2013; 47:106-12. DOI: 10.1016/j.bios.2013.03.008. View

18.

Zhu Z, Lei J, Liu L, Ju H . Label-free electrochemical DNA sensing with a one-target-multitriggered hybridization chain reaction strategy. Analyst. 2013; 138(20):5995-6000. DOI: 10.1039/c3an01212c. View

19.

Freage L, Wang F, Orbach R, Willner I . Multiplexed analysis of genes and of metal ions using enzyme/DNAzyme amplification machineries. Anal Chem. 2014; 86(22):11326-33. DOI: 10.1021/ac5030667. View

20.

Luo X, Hsing I . Electrochemical techniques on sequence-specific PCR amplicon detection for point-of-care applications. Analyst. 2009; 134(10):1957-64. DOI: 10.1039/b912653h. View