PKD1 Mono-allelic Knockout is Sufficient to Trigger Renal Cystogenesis in a Mini-pig Model

Overview

Journal Int J Biol Sci

Specialty Biology

Date 2015 Mar 24

PMID 25798056

Citations 19

Authors

Jin He

Qiuyan Li

Suyun Fang

Ying Guo

Tongxin Liu

Jianhua Ye

Zhengquan Yu

Ran Zhang

Yaofeng Zhao

Xiaoxiang Hu

Xueyuan Bai

Xiangmei Chen

Ning Li

Affiliations

Soon will be listed here.

Abstract

PKD1 and PKD2 mutations could lead to autosomal dominant polycystic kidney disease (ADPKD), which afflicts millions of people worldwide. Due to the marked differences in the lifespan, size, anatomy, and physiology from humans, rodent ADPKD models cannot fully mimic the disease. To obtain a large animal model that recapitulates the disease, we constructed a mini-pig model by mono-allelic knockout (KO) of PKD1 using zinc finger nuclease. The mono-allelic KO pigs had lower PKD1 expression than their wild-type littermates at both the transcriptional and translational levels. After approximately six months, renal cysts appeared and grew progressively in the KO pigs. Histological analysis showed that renal cysts were scatteredly distributed in the mutant pig kidneys and were lined by either cuboidal or flattened epithelial cells. Contrast-enhanced computed tomography confirmed that all of the mutant pigs had renal and hepatic cysts, when they were 11-month-old. Immunohistochemical analysis revealed that most of the cysts were derived from the proximal tubules and collecting ducts. Therefore, the PKD1 mono-allelic knockout is sufficient to trigger renal cystogenesis, and this pig model may provide a platform for future study of renal cyst formation.

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