Rat Pancreatic Islet Cells in Primary Culture: Occurrence of Giant Cells Amenable to Patch Clamping
Overview
Overview
Authors
Authors
Affiliations
Affiliations
Soon will be listed here.
Abstract
Neonatal and adult rat pancreatic islet cells were maintained in dissociated cell culture for up to three weeks. The unexpected occurrence of giant (40-50 micron) cells was noted, some of which reacted positively to an insulin antiserum, indicating the presence of insulin. The giant cells were amenable to study using the extracellular patch clamp technique, which was used to demonstrate a population of membrane channels gating outwardly directed current in these cells.
References
1.
Orci L, LIKE A, Amherdt M, Blondel B, Kanazawa Y, Marliss E
. Monolayer cell culture of neonatal rat pancreas: an ultrastructural and biochemical study of functioning endocrine cells. J Ultrastruct Res. 1973; 43(3):270-97.
DOI: 10.1016/s0022-5320(73)80039-5.
View
2.
Amsterdam A, JAMIESON J
. Structural and functional characterization of isolated pancreatic exocrine cells. Proc Natl Acad Sci U S A. 1972; 69(10):3028-32.
PMC: 389700.
DOI: 10.1073/pnas.69.10.3028.
View
3.
Hellerstrom C, Lewis N, Borg H, Johnson R, Freinkel N
. Method for large-scale isolation of pancreatic islets by tissue culture of fetal rat pancreas. Diabetes. 1979; 28(8):769-76.
DOI: 10.2337/diab.28.8.769.
View
4.
Colquhoun D, Hawkes A
. On the stochastic properties of single ion channels. Proc R Soc Lond B Biol Sci. 1981; 211(1183):205-35.
DOI: 10.1098/rspb.1981.0003.
View
5.
Horn R, Patlak J
. Single channel currents from excised patches of muscle membrane. Proc Natl Acad Sci U S A. 1980; 77(11):6930-4.
PMC: 350405.
DOI: 10.1073/pnas.77.11.6930.
View