Effect of Egg White Combined with Chalcanthite on Lipopolysaccharide Induced Inflammatory Cytokine Expression in RAW 264.7 Cells

Overview

Journal J Pharmacopuncture

Date 2015 Mar 18

PMID 25780629

Authors

Eun-A Choi

Jeung-Won Yoon

Hak-Joo Choi

Dong-Hee Kim

Hwa-Seung Yoo

Affiliations

Soon will be listed here.

Abstract

Aim: Historically, mineral compound herbal medicines have long been used in treatments of immune-related diseases in Korea, China and other Asian countries. In this study, we investigated the anti-inflammatory effect of egg white combined with chalcanthite (IS4) on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.

Methods: RAW 264.7 cells cultured with LPS and various concentrations of IS4 were analyzed to determine the production of pro-inflammatory cytokines and mediators by using enzyme-linked immune sorbent assays (ELISAs).

Results: IS4 concentration inhibited the production of interleukin-1beta (IL-1 β), interleukin-6 (IL-6), and granulocyte -macrophage colony-stimulating factor (GM-CSF) induced by LPS. IS4 at high concentrations (25 and 50㎍/ml) inhibited, in concentration-dependent manner, the expression of tumor necrosis factor-alpha (TNF- α) stimulated by LPS.

Conclusion: IS4 has shown an anti-inflammatory effect in RAW 264.7 cells.

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