Asymmetric Unwrapping of Nucleosomes Under Tension Directed by DNA Local Flexibility

Overview

Journal Cell

Publisher Cell Press

Specialty Cell Biology

Date 2015 Mar 14

PMID 25768909

Citations 161

Authors

Thuy T M Ngo

Qiucen Zhang

Ruobo Zhou

Jaya G Yodh

Taekjip Ha

Affiliations

Soon will be listed here.

Abstract

Dynamics of the nucleosome and exposure of nucleosomal DNA play key roles in many nuclear processes, but local dynamics of the nucleosome and its modulation by DNA sequence are poorly understood. Using single-molecule assays, we observed that the nucleosome can unwrap asymmetrically and directionally under force. The relative DNA flexibility of the inner quarters of nucleosomal DNA controls the unwrapping direction such that the nucleosome unwraps from the stiffer side. If the DNA flexibility is similar on two sides, it stochastically unwraps from either side. The two ends of the nucleosome are orchestrated such that the opening of one end helps to stabilize the other end, providing a mechanism to amplify even small differences in flexibility to a large asymmetry in nucleosome stability. Our discovery of DNA flexibility as a critical factor for nucleosome dynamics and mechanical stability suggests a novel mechanism of gene regulation by DNA sequence and modifications.

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