A Novel and Effective Chromatographic Approach to the Separation of Isoflavone Derivatives from Pueraria Lobata

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2015 Mar 10

PMID 25751785

Citations 2

Authors

Jiang Fu

Wenguang Jing

Weihao Wang

Sha Chen

Jun Zhang

An Liu

Affiliations

Soon will be listed here.

Abstract

A novel and effective chromatographic approach to the separation and purification of isoflavone compounds from Pueraria lobata is described. The method is based on flash chromatography (FC), coupled to preparative high performance liquid chromatography (prep-HPLC) via a six-way valve. The FC step comprised tandem reversed phase columns, pre-packed with MCI gel (Mitsubishi Chemical Corp., Tokyo, Japan) and C18 (Fuji Silysia Chemical Ltd, Osaka, Japan) resin, respectively, and was designed to separate a crude Pueraria lobata extract into several preliminary fractions. Fractions containing the target compounds were then directly injected via the six-way valve into prep-HPLC columns, without further treatment, for final isolation and purification. Nine isoflavonoids were successfully isolated, three through an online mode and the other six through an offline mode. The purities of all compounds exceeded 95.0%, as determined by HPLC with an UV-vis photodiode array detector. The convenience, low solvent consumption, and time-saving advantages of this method offer an attractive and promising approach to the isolation of natural products.

Citing Articles

Isolation of Mirificin and Other Bioactive Isoflavone Glycosides from the Kudzu Root Lyophilisate Using Centrifugal Partition and Flash Chromatographic Techniques.

Maciejewska-Turska M, Pecio L, Zgorka G Molecules. 2022; 27(19).

PMID: 36234764 PMC: 9570587. DOI: 10.3390/molecules27196227.

Puerarin protects vascular smooth muscle cells from oxidized low-density lipoprotein-induced reductions in viability via inhibition of the p38 MAPK and JNK signaling pathways.

Hu Y, Li H, Li R, Wu Z, Yang W, Qu W Exp Ther Med. 2020; 20(6):270.

PMID: 33199995 PMC: 7664596. DOI: 10.3892/etm.2020.9400.

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