Identification and Molecular Characterization of Parkin in Clonorchis Sinensis

Overview

Journal Korean J Parasitol

Specialty Parasitology

Date 2015 Mar 10

PMID 25748711

Citations 2

Authors

Xuelian Bai

Tae Im Kim

Ji-Yun Lee

Fuhong Dai

Sung-Jong Hong

Affiliations

Soon will be listed here.

Abstract

Clonorchis sinensis habitating in the bile duct of mammals causes clonorchiasis endemic in East Asian countries. Parkin is a RING-between-RING protein and has E3-ubiquitin ligase activity catalyzing ubiquitination and degradation of substrate proteins. A cDNA clone of C. sinensis was predicted to encode a polypeptide homologous to parkin (CsParkin) including 5 domains (Ubl, RING0, RING1, IBR, and RING2). The cysteine and histidine residues binding to Zn(2+) were all conserved and participated in formation of tertiary structural RINGs. Conserved residues were also an E2-binding site in RING1 domain and a catalytic cysteine residue in the RING2 domain. Native CsParkin was determined to have an estimated molecular weight of 45.7 kDa from C. sinensis adults by immunoblotting. CsParkin revealed E3-ubiquitin ligase activity and higher expression in metacercariae than in adults. CsParkin was localized in the locomotive and male reproductive organs of C. sinensis adults, and extensively in metacercariae. Parkin has been found to participate in regulating mitochondrial function and energy metabolism in mammalian cells. From these results, it is suggested that CsParkin play roles in energy metabolism of the locomotive organs, and possibly in protein metabolism of the reproductive organs of C. sinensis.

Citing Articles

Characterization of a novel organic solute transporter homologue from Clonorchis sinensis.

Lu Y, Yoo W, Dai F, Lee J, Pak J, Sohn W PLoS Negl Trop Dis. 2018; 12(4):e0006459.

PMID: 29702646 PMC: 5942847. DOI: 10.1371/journal.pntd.0006459.

Molecular and structural characteristics of multidrug resistance-associated protein 7 in Chinese liver fluke Clonorchis sinensis.

Dai F, Yoo W, Lee J, Lu Y, Pak J, Sohn W Parasitol Res. 2017; 116(3):953-962.

PMID: 28058535 DOI: 10.1007/s00436-016-5371-0.

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