Structure and Dynamics of the MKK7-JNK Signaling Complex

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2015 Mar 5

PMID 25737554

Citations 35

Authors

Jaka Kragelj

Andres Palencia

Max H Nanao

Damien Maurin

Guillaume Bouvignies

Martin Blackledge

Malene Ringkjobing Jensen

Affiliations

Soon will be listed here.

Abstract

Signaling specificity in the mitogen-activated protein kinase (MAPK) pathways is controlled by disordered domains of the MAPK kinases (MKKs) that specifically bind to their cognate MAPKs via linear docking motifs. MKK7 activates the c-Jun N-terminal kinase (JNK) pathway and is the only MKK containing three motifs within its regulatory domain. Here, we characterize the conformational behavior and interaction mechanism of the MKK7 regulatory domain. Using NMR spectroscopy, we develop an atomic resolution ensemble description of MKK7, revealing highly diverse intrinsic conformational propensities of the three docking sites, suggesting that prerecognition sampling of the bound-state conformation is not prerequisite for binding. Although the different sites exhibit similar affinities for JNK1, interaction kinetics differ considerably. Importantly, we determine the crystal structure of JNK1 in complex with the second docking site of MKK7, revealing two different binding modes of the docking motif correlating with observations from NMR exchange spectroscopy. Our results provide unique insight into how signaling specificity is regulated by linear motifs and, in general, into the role of conformational disorder in MAPK signaling.

Citing Articles

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