Engineering a Ca⁺⁺⁺-sensitive (bio)sensor from the Pore-module of a Potassium Channel

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2015 Mar 4

PMID 25734643

Citations 2

Authors

Mattia Lorenzo DiFrancesco

Sabrina Gazzarrini

Cristina Arrigoni

Giulia Romani

Gerhard Thiel

Anna Moroni

Affiliations

Soon will be listed here.

Abstract

Signals recorded at the cell membrane are meaningful indicators of the physiological vs. pathological state of a cell and will become useful diagnostic elements in nanomedicine. In this project we present a coherent strategy for the design and fabrication of a bio-nano-sensor that monitors changes in intracellular cell calcium concentration and allows an easy read out by converting the calcium signal into an electrical current in the range of microampere that can be easily measured by conventional cell electrophysiology apparatus.

Citing Articles

Distinct classes of potassium channels fused to GPCRs as electrical signaling biosensors.

Garcia-Fernandez M, Chatelain F, Nury H, Moroni A, Moreau C Cell Rep Methods. 2022; 1(8):None.

PMID: 34977850 PMC: 8688152. DOI: 10.1016/j.crmeth.2021.100119.

Exploring the Viral Channel Kcv Function via Computation.

Andersson A, Kasimova M, Delemotte L J Membr Biol. 2018; 251(3):419-430.

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