The Forgotten Lactogenic Activity of Growth Hormone: Important Implications for Rodent Studies

Overview

Journal Endocrinology

Publisher Oxford University Press

Specialty Endocrinology

Date 2015 Mar 3

PMID 25730109

Citations 10

Authors

Andrzej Bartke

John J Kopchick

Affiliations

Soon will be listed here.

Abstract

Studies of the effects of GH and the mechanisms of its actions frequently use rats or mice and various recombinant human GH preparations. Authors of many of these studies appear unaware of the fact that, in rodents, human GH signals through both GH and prolactin (PRL) receptors; thus, treatment with human GH is equivalent to a combined treatment with GH and PRL. GH receptors and PRL receptors are present in multiple cell types. Importantly, PRL exerts major effects on brain neuroendocrine action, female and male reproduction, metabolism, body composition, immune responses, and a host of other functions; thus, treatment of rodents with recombinant human GH could affect these important physiological parameters.

Citing Articles

Diastolic Dysfunction with Normal Ejection Fraction and Reduced Heart Rate in Mice Expressing Human Growth Hormone and Displaying Signs of Growth Hormone Insufficiency.

Jin Y, Xiang B, Dolinsky V, Kardami E, Cattini P Int J Mol Sci. 2025; 26(1.

PMID: 39796124 PMC: 11719473. DOI: 10.3390/ijms26010269.

The Pattern of GH Action in the Mouse Brain.

Menezes F, Wasinski F, de Souza G, Nunes A, Bernardes E, Dos Santos S Endocrinology. 2024; 165(7).

PMID: 38728240 PMC: 11137758. DOI: 10.1210/endocr/bqae057.

Parental factors that impact the ecology of human mammary development, milk secretion, and milk composition-a report from "Breastmilk Ecology: Genesis of Infant Nutrition (BEGIN)" Working Group 1.

Neville M, Demerath E, Hahn-Holbrook J, Hovey R, Martin-Carli J, McGuire M Am J Clin Nutr. 2023; 117 Suppl 1:S11-S27.

PMID: 37173058 PMC: 10232333. DOI: 10.1016/j.ajcnut.2022.11.026.

Prolactin Action Is Necessary for Parental Behavior in Male Mice.

Smiley K, Brown R, Grattan D J Neurosci. 2022; 42(44):8308-8327.

PMID: 36163141 PMC: 9653282. DOI: 10.1523/JNEUROSCI.0558-22.2022.

Mice with gene alterations in the GH and IGF family.

Qian Y, Berryman D, Basu R, List E, Okada S, Young J Pituitary. 2021; 25(1):1-51.

PMID: 34797529 PMC: 8603657. DOI: 10.1007/s11102-021-01191-y.

References

Karabulut A, Layfield R, Pratten M . The mechanism of growth-promoting effects of prolactin in embryogenesis--links to growth factors. Cells Tissues Organs. 2000; 164(1):2-13. DOI: 10.1159/000016637. View

HARTREE A, Kovacic N, Thomas M . Growth-promoting and luteotrophic activities of human growth hormone. J Endocrinol. 1965; 33(2):249-58. DOI: 10.1677/joe.0.0330249. View

Gala R . Prolactin production by the human anterior pituitary cultured in vitro. J Endocrinol. 1971; 50(4):637-42. DOI: 10.1677/joe.0.0500637. View

Lewis U, Singh R, Seavey B . Human prolactin: isolation and some properties. Biochem Biophys Res Commun. 1971; 44(5):1169-76. DOI: 10.1016/s0006-291x(71)80209-7. View

Posner B, Kelly P, Shiu R, Friesen H . Studies of insulin, growth hormone and prolactin binding: tissue distribution, species variation and characterization. Endocrinology. 1974; 95(2):521-31. DOI: 10.1210/endo-95-2-521. View

Hwang P, Guyda H, Friesen H . Purification of human prolactin. Methods Enzymol. 1975; 37:389-402. DOI: 10.1016/s0076-6879(75)37034-1. View

Bartke A, Goldman B, Bex F, Kelch R, Smith M, Dalterio S . Effects of prolactin on testicular regression and recrudescence in the golden hamster. Endocrinology. 1980; 106(1):167-72. DOI: 10.1210/endo-106-1-167. View

Shiu R, Elsholtz H, Tanaka T, Friesen H, Gout P, BEER C . Receptor-mediated mitogenic action of prolactin in a rat lymphoma cell line. Endocrinology. 1983; 113(1):159-65. DOI: 10.1210/endo-113-1-159. View

Matt K, Bartke A, Soares M, Talamantes F, Hebert A, Hogan M . Does prolactin modify testosterone feedback in the hamster? Suppression of plasma prolactin inhibits photoperiod-induced decreases in testosterone feedback sensitivity. Endocrinology. 1984; 115(6):2098-103. DOI: 10.1210/endo-115-6-2098. View

10.

Bartke A, Steger R, Hodges S, Parkening T, Collins T, Yun J . Infertility in transgenic female mice with human growth hormone expression: evidence for luteal failure. J Exp Zool. 1988; 248(1):121-4. DOI: 10.1002/jez.1402480116. View

11.

Cunningham B, Wells J . High-resolution epitope mapping of hGH-receptor interactions by alanine-scanning mutagenesis. Science. 1989; 244(4908):1081-5. DOI: 10.1126/science.2471267. View

12.

Nickel B, Kardami E, Cattini P . The human placental growth hormone variant is mitogenic for rat lymphoma Nb2 cells. Endocrinology. 1990; 126(2):971-6. DOI: 10.1210/endo-126-2-971. View

13.

Brem G, Wanke R, Wolf E, Buchmuller T, Muller M, Brenig B . Multiple consequences of human growth hormone expression in transgenic mice. Mol Biol Med. 1989; 6(6):531-47. View

14.

Cunningham B, Bass S, Fuh G, Wells J . Zinc mediation of the binding of human growth hormone to the human prolactin receptor. Science. 1990; 250(4988):1709-12. DOI: 10.1126/science.2270485. View

15.

Steger R, Bartke A, Parkening T, Collins T, Buonomo F, Tang K . Effects of heterologous growth hormones on hypothalamic and pituitary function in transgenic mice. Neuroendocrinology. 1991; 53(4):365-72. DOI: 10.1159/000125743. View

16.

Milton S, Cecim M, Li Y, Yun J, Wagner T, Bartke A . Transgenic female mice with high human growth hormone levels are fertile and capable of normal lactation without having been pregnant. Endocrinology. 1992; 131(1):536-8. DOI: 10.1210/endo.131.1.1612034. View

17.

Vashdi D, Elberg G, Sakal E, Gertler A . Biological activity of bovine placental lactogen in 3T3-F442A preadipocytes is mediated through a somatogenic receptor. FEBS Lett. 1992; 305(2):101-4. DOI: 10.1016/0014-5793(92)80873-f. View

18.

Gertler A, Hauser S, Sakal E, Vashdi D, Staten N, Freeman J . Preparation, purification, and determination of the biological activities of 12 N terminus-truncated recombinant analogues of bovine placental lactogen. J Biol Chem. 1992; 267(18):12655-9. View

19.

Bartke A, Naar E, Johnson L, May M, Cecim M, Yun J . Effects of expression of human or bovine growth hormone genes on sperm production and male reproductive performance in four lines of transgenic mice. J Reprod Fertil. 1992; 95(1):109-18. DOI: 10.1530/jrf.0.0950109. View

20.

Fiddes R, Brandon M, Adams T . Functional expression of an ovine growth hormone receptor in transfected Chinese hamster ovary cells. Mol Cell Endocrinol. 1992; 86(1-2):37-47. DOI: 10.1016/0303-7207(92)90173-4. View