Preliminary Estimation of Deoxynivalenol Excretion Through a 24 H Pilot Study

Overview

Journal Toxins (Basel)

Publisher MDPI

Specialty Toxicology

Date 2015 Feb 28

PMID 25723325

Citations 11

Authors

Yelko Rodriguez-Carrasco

Jordi Manes

Houda Berrada

Guillermina Font

Affiliations

Soon will be listed here.

Abstract

A duplicate diet study was designed to explore the occurrence of 15 Fusarium mycotoxins in the 24 h-diet consumed by one volunteer as well as the levels of mycotoxins in his 24 h-collected urine. The employed methodology involved solvent extraction at high ionic strength followed by dispersive solid phase extraction and gas chromatography determination coupled to mass spectrometry in tandem. Satisfactory results in method performance were achieved. The method's accuracy was in a range of 68%-108%, with intra-day relative standard deviation and inter-day relative standard deviation lower than 12% and 15%, respectively. The limits of quantitation ranged from 0.1 to 8 µg/Kg. The matrix effect was evaluated and matrix-matched calibrations were used for quantitation. Only deoxynivalenol (DON) was quantified in both food and urine samples. A total DON daily intake amounted to 49.2 ± 5.6 µg whereas DON daily excretion of 35.2 ± 4.3 µg was determined. DON daily intake represented 68.3% of the established DON provisional maximum tolerable daily intake (PMTDI). Valuable preliminary information was obtained as regards DON excretion and needs to be confirmed in large-scale monitoring studies.

Citing Articles

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The Presence of Mycotoxins in Human Amniotic Fluid.

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