A New Device for Step-down Inhibitory Avoidance Task--effects of Low and High Frequency in a Novel Device for Passive Inhibitory Avoidance Task That Avoids Bioimpedance Variations

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2015 Feb 24

PMID 25706879

Citations 6

Authors

Gilvan Luiz Borba Filho

Kamila Cagliari Zenki

Eduardo Kalinine

Suelen Baggio

Leticia Pettenuzzo

Eduardo Rigon Zimmer

Simone Nardin Weis

Maria Elisa Calcagnotto

Diogo Onofre de Souza

Affiliations

Soon will be listed here.

Abstract

Background: Step-down inhibitory avoidance task has been widely used to evaluate aversive memory, but crucial parameters inherent to traditional devices that may influence the behavior analysis (as stimulus frequency, animal's bioimpedance) are frequently neglected.

New Method: We developed a new device for step-down inhibitory avoidance task by modifying the shape and distribution of the stainless steel bars in the box floor where the stimuli are applied. The bars are 2 mm wide, with rectangular shape, arranged in pairs at intervals of 1cm from the next pairs. Each pair makes an electrical dipole where the polarity inverts after each pulse. This device also presents a component that acquires and records the exact current received by the animal foot and precisely controls the frequency of stimulus applied during the entire experiment.

Result: Different from conventional devices, this new apparatus increases the contact surface with bars and animal's paws, allowing the electric current pass through the animal's paws only, drastically reducing the influence of animal's bioimpedance. The analysis of recorded data showed that the current received by the animal was practically the same as applied, independent of the animal's body composition. Importantly, the aversive memory was observed at specific stimuli intensity and frequency (0.35 or 0.5 mA at 62 and 125 Hz but not at 0.20 mA or 20 Hz). Moreover, with this device it was possible to observe the well-known step-down inhibitory avoidance task memory impairment induced by guanosine.

Conclusion: This new device offers a substantial improvement for behavioral analysis in step-down inhibitory avoidance task and allows us to precisely compare data from different animals with distinct body composition.

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References

CARRAN A, Yeudall L, Royce J . VOLTAGE LEVEL AND SKIN RESISTANCE IN AVOIDANCE CONDITIONING OF INBRED STRAINS OF MICE. J Comp Physiol Psychol. 1964; 58:427-30. DOI: 10.1037/h0046774. View

Izquierdo I, Medina J, Vianna M, Izquierdo L, Barros D . Separate mechanisms for short- and long-term memory. Behav Brain Res. 1999; 103(1):1-11. DOI: 10.1016/s0166-4328(99)00036-4. View

Vinade E, Izquierdo I, Lara D, Schmidt A, Souza D . Oral administration of guanosine impairs inhibitory avoidance performance in rats and mice. Neurobiol Learn Mem. 2004; 81(2):137-43. DOI: 10.1016/j.nlm.2003.12.003. View

Jiang S, Bendjelloul F, Ballerini P, DAlimonte I, Nargi E, Jiang C . Guanosine reduces apoptosis and inflammation associated with restoration of function in rats with acute spinal cord injury. Purinergic Signal. 2008; 3(4):411-21. PMC: 2072916. DOI: 10.1007/s11302-007-9079-6. View

Petronilho F, Perico S, Vuolo F, Mina F, Constantino L, Comim C . Protective effects of guanosine against sepsis-induced damage in rat brain and cognitive impairment. Brain Behav Immun. 2012; 26(6):904-10. DOI: 10.1016/j.bbi.2012.03.007. View

Perry M, Dias R, Carrasco M, Izquierdo I . Step-down inhibitory avoidance training and beta-endorphin-like immunoreactivity of rat hypothalamus and plasma. Braz J Med Biol Res. 1983; 16(4):339-43. View

Bevilaqua L, da Silva W, Medina J, Izquierdo I, Cammarota M . Extinction and reacquisition of a fear-motivated memory require activity of the Src family of tyrosine kinases in the CA1 region of the hippocampus. Pharmacol Biochem Behav. 2005; 81(1):139-45. DOI: 10.1016/j.pbb.2005.03.005. View

Jerusalinsky D, Ferreira M, Walz R, Silva R, Bianchin M, Ruschel A . Amnesia by post-training infusion of glutamate receptor antagonists into the amygdala, hippocampus, and entorhinal cortex. Behav Neural Biol. 1992; 58(1):76-80. DOI: 10.1016/0163-1047(92)90982-a. View

Wahlsten D . Phenotypic and genetic relations between initial response to electric shock and rate of avoidance learning in mice. Behav Genet. 1972; 2(2):211-40. DOI: 10.1007/BF01065691. View

10.

Schmidt A, Lara D, de Faria Maraschin J, da Silveira Perla A, Onofre Souza D . Guanosine and GMP prevent seizures induced by quinolinic acid in mice. Brain Res. 2000; 864(1):40-3. DOI: 10.1016/s0006-8993(00)02106-5. View

11.

Quevedo J, Vianna M, Roesler R, De-Paris F, Izquierdo I, Rose S . Two time windows of anisomycin-induced amnesia for inhibitory avoidance training in rats: protection from amnesia by pretraining but not pre-exposure to the task apparatus. Learn Mem. 2000; 6(6):600-7. PMC: 311311. DOI: 10.1101/lm.6.6.600. View

12.

Nazari-Serenjeh F, Rezayof A . Cooperative interaction between the basolateral amygdala and ventral tegmental area modulates the consolidation of inhibitory avoidance memory. Prog Neuropsychopharmacol Biol Psychiatry. 2012; 40:54-61. DOI: 10.1016/j.pnpbp.2012.10.003. View

13.

Lara D, Schmidt A, Frizzo M, Burgos J, Ramirez G, Souza D . Effect of orally administered guanosine on seizures and death induced by glutamatergic agents. Brain Res. 2001; 912(2):176-80. DOI: 10.1016/s0006-8993(01)02734-2. View

14.

Galindo L, Garin-Aguilar M, Medina A, Serafin N, Quirarte G, Prado-Alcala R . Acquisition and retention of enhanced active avoidance are unaffected by interference with serotonergic activity. Behav Brain Res. 2008; 195(1):153-8. DOI: 10.1016/j.bbr.2008.01.011. View

15.

Bianchin M, Walz R, Ruschel A, Zanatta M, Silva R, Bueno E Silva M . Memory expression is blocked by the infusion of CNQX into the hippocampus and/or the amygdala up to 20 days after training. Behav Neural Biol. 1993; 59(2):83-6. DOI: 10.1016/0163-1047(93)90782-d. View

16.

Walz R, Roesler R, Quevedo J, SantAnna M, Madruga M, Rodrigues C . Time-dependent impairment of inhibitory avoidance retention in rats by posttraining infusion of a mitogen-activated protein kinase kinase inhibitor into cortical and limbic structures. Neurobiol Learn Mem. 2000; 73(1):11-20. DOI: 10.1006/nlme.1999.3913. View

17.

Izquierdo I, Quillfeldt J, Zanatta M, Quevedo J, Schaeffer E, Schmitz P . Sequential role of hippocampus and amygdala, entorhinal cortex and parietal cortex in formation and retrieval of memory for inhibitory avoidance in rats. Eur J Neurosci. 1997; 9(4):786-93. DOI: 10.1111/j.1460-9568.1997.tb01427.x. View

18.

Skalicky M, Narath E, Viidik A . Housing conditions influence the survival and body composition of ageing rats. Exp Gerontol. 2001; 36(1):159-70. DOI: 10.1016/s0531-5565(00)00174-1. View

19.

Glick S, Greenstein S, Goldfarb J . Increased electrical impedance of mice following administration of scopolamine. Behav Biol. 1973; 9(6):771-5. DOI: 10.1016/s0091-6773(73)80138-5. View

20.

Hu L, Maslanik T, Zerebeckyj M, Plato C . Evaluation of bioimpedance spectroscopy for the measurement of body fluid compartment volumes in rats. J Pharmacol Toxicol Methods. 2012; 65(2):75-82. DOI: 10.1016/j.vascn.2012.02.001. View