Ameliorating Effects of Ethanol Extract of Fructus Mume on Scopolamine-Induced Memory Impairment in Mice

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2015 Feb 24

PMID 25705233

Citations 12

Authors

Min-Soo Kim

Won Kyung Jeon

Kye Wan Lee

Yu Hwa Park

Jung-Soo Han

Affiliations

Soon will be listed here.

Abstract

We previously reported that Fructus mume (F. mume) extract shows protective effects on memory impairments and anti-inflammatory effects induced by chronic cerebral hypoperfusion. Neurodegeneration of basal cholinergic neurons is also observed in the brain with chronic cerebral hypoperfusion. Therefore, the present study was conducted to examine whether F. mume extracts enhance cognitive function via the action of cholinergic neuron using a scopolamine-induced animal model of memory impairments. F. mume (50, 100, or 200 mg/kg) was administered to C57BL/6 mice for 14 days (days 1-14) and memory impairment was induced by scopolamine (1 mg/kg), a muscarinic receptor antagonist for 7 days (days 8-14). Spatial memory was assessed using Morris water maze and hippocampal level of acetylcholinesterase (AChE) and choline acetyltransferase (ChAT) was examined by ELISA and immunoblotting. Mice that received scopolamine alone showed impairments in acquisition and retention in Morris water maze task and increased activity of AChE in the hippocampus. Mice that received F. mume and scopolamine showed no scopolamine-induced memory impairment and increased activity of AChE. In addition, treatments of F. mume increased ChAT expression in the hippocampus. These results indicated that F. mume might enhance cognitive function via action of cholinergic neurons.

Citing Articles

Acetylcholinesterase Inhibitors in the Treatment of Neurodegenerative Diseases and the Role of Acetylcholinesterase in their Pathogenesis.

Walczak-Nowicka L, Herbet M Int J Mol Sci. 2021; 22(17).

PMID: 34502198 PMC: 8430571. DOI: 10.3390/ijms22179290.

Effects of chronic tramadol administration on cognitive flexibility in mice.

Attoh-Mensah E, Leger M, Loggia G, Freret T, Chavoix C, Schumann-Bard P Psychopharmacology (Berl). 2021; 238(10):2883-2893.

PMID: 34173033 DOI: 10.1007/s00213-021-05903-x.

Comprehensive Review of Phytochemical Constituents, Pharmacological Properties, and Clinical Applications of .

Gong X, Tang Y, Song Y, Du G, Li J Front Pharmacol. 2021; 12:679378.

PMID: 34122104 PMC: 8195681. DOI: 10.3389/fphar.2021.679378.

Pharmacological Treatment of Vascular Dementia: A Molecular Mechanism Perspective.

Kuang H, Zhou Z, Zhu Y, Wan Z, Yang M, Hong F Aging Dis. 2021; 12(1):308-326.

PMID: 33532143 PMC: 7801279. DOI: 10.14336/AD.2020.0427.

Wenyang Jieyu Decoction Alleviates Depressive Behavior in the Rat Model of Depression via Regulation of the Intestinal Microbiota.

Feng Z, Ma X, Meng S, Wang H, Zhou X, Shi M Evid Based Complement Alternat Med. 2020; 2020:3290450.

PMID: 32774410 PMC: 7396094. DOI: 10.1155/2020/3290450.

References

Kwon H, Kwon Y, Kwon D, Lee J . Evaluation of antibacterial effects of a combination of Coptidis Rhizoma, Mume Fructus, and Schizandrae Fructus against Salmonella. Int J Food Microbiol. 2008; 127(1-2):180-3. DOI: 10.1016/j.ijfoodmicro.2008.06.020. View

Squire L, Zola S . Structure and function of declarative and nondeclarative memory systems. Proc Natl Acad Sci U S A. 1996; 93(24):13515-22. PMC: 33639. DOI: 10.1073/pnas.93.24.13515. View

Choi B, Kwon K, Park S, Jeon W, Han S, Kim H . Alternations of Septal-hippocampal System in the Adult Wistar Rat with Spatial Memory Impairments Induced by Chronic Cerebral Hypoperfusion. Exp Neurobiol. 2011; 20(2):92-9. PMC: 3213701. DOI: 10.5607/en.2011.20.2.92. View

Giacobini E . Cholinesterase inhibitors: new roles and therapeutic alternatives. Pharmacol Res. 2004; 50(4):433-40. DOI: 10.1016/j.phrs.2003.11.017. View

Oda Y . Choline acetyltransferase: the structure, distribution and pathologic changes in the central nervous system. Pathol Int. 1999; 49(11):921-37. DOI: 10.1046/j.1440-1827.1999.00977.x. View

Giovannini M, Spignoli G, Carla V, Pepeu G . A decrease in brain catecholamines prevents oxiracetam antagonism of the effects of scopolamine on memory and brain acetylcholine. Pharmacol Res. 1991; 24(4):395-405. DOI: 10.1016/1043-6618(91)90044-x. View

Dawson G, Heyes C, Iversen S . Pharmacological mechanisms and animal models of cognition. Behav Pharmacol. 1992; 3(4):285-297. View

Tsuchiya M, Sako K, Yura S, Yonemasu Y . Cerebral blood flow and histopathological changes following permanent bilateral carotid artery ligation in Wistar rats. Exp Brain Res. 1992; 89(1):87-92. DOI: 10.1007/BF00229004. View

Huang L, Su T, Li X . Natural products as sources of new lead compounds for the treatment of Alzheimer's disease. Curr Top Med Chem. 2013; 13(15):1864-78. DOI: 10.2174/15680266113139990142. View

10.

Gallagher M, Burwell R, Burchinal M . Severity of spatial learning impairment in aging: development of a learning index for performance in the Morris water maze. Behav Neurosci. 1993; 107(4):618-26. DOI: 10.1037//0735-7044.107.4.618. View

11.

Eichenbaum H, Otto T, Cohen N . The hippocampus--what does it do?. Behav Neural Biol. 1992; 57(1):2-36. DOI: 10.1016/0163-1047(92)90724-i. View

12.

Bartus R, Dean 3rd R, Beer B, Lippa A . The cholinergic hypothesis of geriatric memory dysfunction. Science. 1982; 217(4558):408-14. DOI: 10.1126/science.7046051. View

13.

Shin E, Hur H, Sung M, Park J, Yang H, Kim M . Ethanol extract of the Prunus mume fruits stimulates glucose uptake by regulating PPAR-γ in C2C12 myotubes and ameliorates glucose intolerance and fat accumulation in mice fed a high-fat diet. Food Chem. 2013; 141(4):4115-21. DOI: 10.1016/j.foodchem.2013.06.059. View

14.

Lee S, Kim J, Seo S, Choi B, Han J, Lee K . Sulforaphane alleviates scopolamine-induced memory impairment in mice. Pharmacol Res. 2014; 85:23-32. DOI: 10.1016/j.phrs.2014.05.003. View

15.

Yan X, Lee S, Li W, Sun Y, Yang S, Jang H . Evaluation of the antioxidant and anti-osteoporosis activities of chemical constituents of the fruits of Prunus mume. Food Chem. 2014; 156:408-15. DOI: 10.1016/j.foodchem.2014.01.078. View

16.

Hirokawa S, Nose M, Ishige A, Amagaya S, Oyama T, Ogihara Y . Effect of Hachimi-jio-gan on scopolamine-induced memory impairment and on acetylcholine content in rat brain. J Ethnopharmacol. 1996; 50(2):77-84. DOI: 10.1016/0378-8741(95)01332-6. View

17.

Abe E . Reversal effect of DM-9384 on scopolamine-induced acetylcholine depletion in certain regions of the mouse brain. Psychopharmacology (Berl). 1991; 105(3):310-6. DOI: 10.1007/BF02244423. View

18.

Dong Z, Fu A . Prevention of age-related memory deficit in transgenic mice by human choline acetyltransferase. Eur J Pharmacol. 2012; 683(1-3):174-8. DOI: 10.1016/j.ejphar.2012.03.009. View

19.

Tomimoto H, Ihara M, Wakita H, Ohtani R, Lin J, Akiguchi I . Chronic cerebral hypoperfusion induces white matter lesions and loss of oligodendroglia with DNA fragmentation in the rat. Acta Neuropathol. 2003; 106(6):527-34. DOI: 10.1007/s00401-003-0749-3. View

20.

Jeon W, Ma J, Choi B, Han S, Jin Q, Hwang B . Effects of Fructus mume Extract on MAPK and NF-κB Signaling and the Resultant Improvement in the Cognitive Deficits Induced by Chronic Cerebral Hypoperfusion. Evid Based Complement Alternat Med. 2013; 2012:450838. PMC: 3546564. DOI: 10.1155/2012/450838. View