Guidelines for Assessing Mouse Endothelial Function Via Ultrasound Imaging: a Report from the International Society Of Cardiovascular Translational Research

Overview

Journal J Cardiovasc Transl Res

Publisher Springer

Specialty Cardiology & Vascular Diseases

Date 2015 Feb 22

PMID 25701375

Citations 6

Authors

Hua-Ting Wang

Zhen Shan

Wen Li

Maoping Chu

Jian Yang

Dan Yi

Jiaxin Zhan

Zu-Yi Yuan

Sudhanshu Raikwar

Shenming Wang

Chunxiang Zhang

Affiliations

Soon will be listed here.

Abstract

The study is to establish a novel method to determine the endothelial function in mouse carotid arteries in vivo by using high-resolution ultrasound images. Atherosclerosis in carotid arteries is induced in ApoE(-/-) mice with a Western diet. The ultrasound of the ventral neck generates clear pictures of the common carotid arteries. Acetylcholine at the range from 5 to 20 μg/kg/min (iv) is able to induce a dose-dependent relaxation as shown by the increased diameter of these normal mouse carotid arteries, which is impaired in atherosclerotic arteries. The endothelial function determined by ultrasound images in vivo matches well with that determined in isolated carotid arterial rings in vitro. All animals survived after the endothelial function measurement. In this study, we have established a standard method to determine the mouse endothelial function in vivo. It is a reliable, safe, and survival method that could be used repetitively in mouse arteries.

Citing Articles

Leukocyte-Specific Morrbid Promotes Leukocyte Differentiation and Atherogenesis.

Xiang D, Jiang L, Yuan Q, Yu Y, Liu R, Chen M Research (Wash D C). 2023; 6:0187.

PMID: 37426471 PMC: 10325668. DOI: 10.34133/research.0187.

Optoacoustic Imaging Offers New Insights into In Vivo Human Skin Vascular Physiology.

Monteiro Rodrigues L, Granja T, de Andrade S Life (Basel). 2022; 12(10).

PMID: 36295063 PMC: 9605317. DOI: 10.3390/life12101628.

Bone marrow endothelial dysfunction promotes myeloid cell expansion in cardiovascular disease.

Rohde D, Vandoorne K, Lee I, Grune J, Zhang S, McAlpine C Nat Cardiovasc Res. 2022; 1(1):28-44.

PMID: 35747128 PMC: 9216333. DOI: 10.1038/s44161-021-00002-8.

Multi-Scale Imaging of Vascular Pathologies in Cardiovascular Disease.

Tiwari A, Elgrably B, Saar G, Vandoorne K Front Med (Lausanne). 2022; 8:754369.

PMID: 35071257 PMC: 8766766. DOI: 10.3389/fmed.2021.754369.

Preclinical techniques to investigate exercise training in vascular pathophysiology.

Sangha G, Goergen C, Prior S, Ranadive S, Clyne A Am J Physiol Heart Circ Physiol. 2021; 320(4):H1566-H1600.

PMID: 33385323 PMC: 8260379. DOI: 10.1152/ajpheart.00719.2020.

References

Garcia M, Lima P, Correia L . Prognostic value of endothelial function in patients with atherosclerosis: systematic review. Arq Bras Cardiol. 2012; 99(3):857-65. DOI: 10.1590/s0066-782x2012005000078. View

Sima A, Stancu C, Simionescu M . Vascular endothelium in atherosclerosis. Cell Tissue Res. 2008; 335(1):191-203. DOI: 10.1007/s00441-008-0678-5. View

Lockowandt U, Liska J, Franco-Cereceda A . Short ischemia causes endothelial dysfunction in porcine coronary vessels in an in vivo model. Ann Thorac Surg. 2001; 71(1):265-9. DOI: 10.1016/s0003-4975(00)02253-0. View

Faulx M, Wright A, Hoit B . Detection of endothelial dysfunction with brachial artery ultrasound scanning. Am Heart J. 2003; 145(6):943-51. DOI: 10.1016/S0002-8703(03)00097-8. View

Jawien J, Nastalek P, Korbut R . Mouse models of experimental atherosclerosis. J Physiol Pharmacol. 2004; 55(3):503-17. View

Guo Y, Zhang C, Du X, Nair U, Yoo T . Morphological and functional alterations of the cochlea in apolipoprotein E gene deficient mice. Hear Res. 2005; 208(1-2):54-67. DOI: 10.1016/j.heares.2005.05.010. View

Mudau M, Genis A, Lochner A, Strijdom H . Endothelial dysfunction: the early predictor of atherosclerosis. Cardiovasc J Afr. 2012; 23(4):222-31. PMC: 3721957. DOI: 10.5830/CVJA-2011-068. View

Zadelaar S, Kleemann R, Verschuren L, de Vries-van der Weij J, van der Hoorn J, Princen H . Mouse models for atherosclerosis and pharmaceutical modifiers. Arterioscler Thromb Vasc Biol. 2007; 27(8):1706-21. DOI: 10.1161/ATVBAHA.107.142570. View

Higashi Y, Kihara Y, Noma K . Endothelial dysfunction and hypertension in aging. Hypertens Res. 2012; 35(11):1039-47. DOI: 10.1038/hr.2012.138. View

10.

Lesniewski L, Connell M, Durrant J, Folian B, Anderson M, Donato A . B6D2F1 Mice are a suitable model of oxidative stress-mediated impaired endothelium-dependent dilation with aging. J Gerontol A Biol Sci Med Sci. 2009; 64(1):9-20. PMC: 2691198. DOI: 10.1093/gerona/gln049. View

11.

Wang H, Zhang W, Zhu C, Bucher C, Blazar B, Zhang C . Inactivation of the adenosine A2A receptor protects apolipoprotein E-deficient mice from atherosclerosis. Arterioscler Thromb Vasc Biol. 2009; 29(7):1046-52. PMC: 2724834. DOI: 10.1161/ATVBAHA.109.188839. View

12.

Chen X, Zhang H, McAfee S, Zhang C . The reciprocal relationship between adiponectin and LOX-1 in the regulation of endothelial dysfunction in ApoE knockout mice. Am J Physiol Heart Circ Physiol. 2010; 299(3):H605-12. PMC: 2944476. DOI: 10.1152/ajpheart.01096.2009. View

13.

Georgescu A, Alexandru N, Constantinescu A, Titorencu I, Popov D . The promise of EPC-based therapies on vascular dysfunction in diabetes. Eur J Pharmacol. 2011; 669(1-3):1-6. DOI: 10.1016/j.ejphar.2011.07.035. View

14.

Landmesser U, Drexler H . Endothelial function and hypertension. Curr Opin Cardiol. 2007; 22(4):316-20. DOI: 10.1097/HCO.0b013e3281ca710d. View