Comparison of Human Adipose-derived Stem Cells and Chondroitinase ABC Transplantation on Locomotor Recovery in the Contusion Model of Spinal Cord Injury in Rats

Overview

Journal Iran J Basic Med Sci

Publisher Mashhad University of Medical Sciences

Specialty General Medicine

Date 2015 Feb 19

PMID 25691946

Citations 14

Authors

Arash Sarveazad

Mehrdad Bakhtiari

Asrin Babahajian

Atusa Janzade

Ali Fallah

Fateme Moradi

Mansoureh Soleimani

Mohammadreza Younesi

Farjam Goudarzi

Mohammad Taghi Joghataei

Affiliations

Soon will be listed here.

Abstract

Objectives: Spinal cord injury (SCI) is one of the most serious clinical diseases and its treatment has been a subject of interest to researchers. There are two important therapeutic strategies in the treatment of SCI: replacing lost tissue cells through cells implantation and scar elimination. Therefore, in this study we used human adipose-derived stem cells (hADSCs) implantation and injection of Chondroitinase ABC. Aim of present study was to answer to this question: which one is more efficient for Improvement of locomotor recovery after SCI in rat? Transplantation of hADSCs or injection of ChABC.

Materials And Methods: The spinal cord of rats was injured by contusion using a weight-drop at the level of T8-9, the hADSCs and Chondroitinase ABC were infused in to the spinal cord tissue after injury. BBB test was performed and recorded for each animal weekly for 8 weeks. After the 8(th) weeks, Serial cross-sections were stained with cresyl violet and examined under a light microscope and area of cavity in the spinal cord was measured.

Results: At 8(th) weeks after injection, hADSCs and ChABC significantly promote locomotor function (P<0.01) and spinal cords of hADSCs and ChABC group had cavities much smaller than those of the control group (P<0.001).

Conclusion: Results of the present study shows dealing with inappropriate neuro-inhibitory environment and glial scar by ChABC have equal role compare to cell therapy (with hADSCs) for improving motor function after SCI and this result in adoption of proper therapeutic strategies for SCI intervention is important.

Citing Articles

Chondroitinase ABC Administration in Locomotion Recovery After Spinal Cord Injury: A Systematic Review and Meta-analysis.

Yousefifard M, Janzadeh A, Ali K, Vazirizadeh-Mahabadi M, Sarveazad A, Madani Neishaboori A Basic Clin Neurosci. 2023; 13(5):609-624.

PMID: 37313020 PMC: 10258590. DOI: 10.32598/bcn.2021.1422.1.

Trophic and immunomodulatory effects of adipose tissue derived stem cells in a preclinical murine model of endometriosis.

Hirakawa T, Yotsumoto F, Shirasu N, Kiyoshima C, Urushiyama D, Yoshikawa K Sci Rep. 2022; 12(1):8031.

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Laparoscopic adipose-derived stem cell harvest technique with bipolar sealing device: Outcome in 12 dogs.

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Efficacy of adipose tissue-derived stem cells in locomotion recovery after spinal cord injury: a systematic review and meta-analysis on animal studies.

Alavi S, Madani Neishaboori A, Hossein H, Sarveazad A, Yousefifard M Syst Rev. 2021; 10(1):213.

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Combined treatment with enteric neural stem cells and chondroitinase ABC reduces spinal cord lesion pathology.

Jevans B, James N, Burnside E, McCann C, Thapar N, Bradbury E Stem Cell Res Ther. 2021; 12(1):10.

PMID: 33407795 PMC: 7789480. DOI: 10.1186/s13287-020-02031-9.

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