The Influence of Enrofloxacin, Florfenicol, Ceftiofur and E. Coli LPS Interaction on T and B Cells Subset in Chicks

This study aimed to investigate the influence of enrofloxacin, florfenicol, ceftiofur and E. coli LPS interaction on T and B subsets in thymus and spleen of newly-hatched chicks. A 126, 1-day-old chicks were administered enrofloxacin, florfenicol or ceftiofur in recommended doses according to the currently treatment schedule advises. E .coli LPS was given intravenously once at the dose of 200 μg kg(-1) BW on the 2nd day of experiment (d. e.). On the 6th and the 14th d. e. thymus and spleens were subjected to flow cytometry investigation.The most significant changes were demonstrated in spleen. The antibiotics administration decreased the percentage of B and T cells subset. Moreover, this suppressive effect was enhanced by E. coli LPS administration. On the 6th d. e. the percentage of CD3(+)TCRγδ(-), CD3(+)TCRγδ(+), CD4(+)CD8(-), CD4(-)CD8(+) decreased significantly after ceftiofur and LPS treatment. A lower percentage of CD3(+)TCRγδ(-), CD4(-)CD8(+) and CD3(+)TCRγδ(+) was observed in enrofloxacine and LPS treated group. The decrease percentage of CD3(+)TCRγδ(+)cells and Bu-1(+) was found after florfenicol and LPS treatment. On the 14th d. e. a decreased percentage of CD4(+)CD8(-) and increased percentage of CD4(-)CD8(+) cells was shown in ceftiofur or enrofloxacine and LPS treated groups. In addition decreased percentage of CD3(+)TCRγδ(+) was found in all antibiotic and LPS treated groups.In this study, it was shown that enrofloxacine, florfenicol, ceftiofur treatment may change the proportions among lymphocytes subset and might have an impact on the immune response to bacterial endotoxins in chicks.