Transcriptional Profiling of Cutaneous MRGPRD Free Nerve Endings and C-LTMRs

Overview

Journal Cell Rep

Publisher Cell Press

Specialties Biology
Cell Biology
Molecular Biology

Date 2015 Feb 17

PMID 25683706

Citations 29

Authors

Ana Reynders

Annabelle Mantilleri

Pascale Malapert

Stephanie Rialle

Sabine Nidelet

Sophie Laffray

Corinne Beurrier

Emmanuel Bourinet

Aziz Moqrich

Affiliations

Soon will be listed here.

Abstract

Cutaneous C-unmyelinated MRGPRD free nerve endings and C-LTMRs innervating hair follicles convey two opposite aspects of touch sensation: a sensation of pain and a sensation of pleasant touch. The molecular mechanisms underlying these diametrically opposite functions are unknown. Here, we used a mouse model that genetically marks C-LTMRs and MRGPRD neurons in combination with fluorescent cell surface labeling, flow cytometry, and RNA deep-sequencing technology (RNA-seq). Cluster analysis of RNA-seq profiles of the purified neuronal subsets revealed 486 and 549 genes differentially expressed in MRGPRD-expressing neurons and C-LTMRs, respectively. We validated 48 MRGPD- and 68 C-LTMRs-enriched genes using a triple-staining approach, and the Ca3.3 channel, found to be exclusively expressed in C-LTMRs, was validated using electrophysiology. Our study greatly expands the molecular characterization of C-LTMRs and suggests that this particular population of neurons shares some molecular features with Aβ and Aδ low-threshold mechanoreceptors.

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