Variola Virus-specific Diagnostic Assays: Characterization, Sensitivity, and Specificity

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2015 Feb 13

PMID 25673790

Citations 7

Authors

Ashley V Kondas

Victoria A Olson

Yu Li

Jason Abel

Miriam Laker

Laura Rose

Kimberly Wilkins

Jonathan Turner

Richard Kline

Inger K Damon

Affiliations

Soon will be listed here.

Abstract

A public health response relies upon rapid and reliable confirmation of disease by diagnostic assays. Here, we detail the design and validation of two variola virus-specific real-time PCR assays, since previous assays cross-reacted with newly identified cowpox viruses. The assay specificity must continually be reassessed as other closely related viruses are identified.

Citing Articles

Teaching a new mouse old tricks: Humanized mice as an infection model for Variola virus.

Hutson C, Kondas A, Ritter J, Reed Z, Ostergaard S, Morgan C PLoS Pathog. 2021; 17(9):e1009633.

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Are We Prepared in Case of a Possible Smallpox-Like Disease Emergence?.

Olson V, Shchelkunov S Viruses. 2020; 9(9).

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The origins and genomic diversity of American Civil War Era smallpox vaccine strains.

Duggan A, Klunk J, Porter A, Dhody A, Hicks R, Smith G Genome Biol. 2020; 21(1):175.

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Use of live Variola virus to determine whether CAST/EiJ mice are a suitable surrogate animal model for human smallpox.

Gallardo-Romero N, Hutson C, Carroll D, Kondas A, Salzer J, Dietz-Ostergaard S Virus Res. 2019; 275:197772.

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Bayesian Phylogeography and Pathogenic Characterization of Smallpox Based on HA, ATI, and CrmB Genes.

Adam D, Scotch M, MacIntyre C Mol Biol Evol. 2018; 35(11):2607-2617.

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