Characterization of Borrelia Burgdorferi Isolates by Restriction Endonuclease Analysis and DNA Hybridization

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1989 Apr 1

PMID 2566622

Citations 29

Authors

R B Lefebvre

G C Perng

R C Johnson

Affiliations

Soon will be listed here.

Abstract

Genomes of several Borrelia burgdorferi isolates from North America and Europe were characterized by restriction endonuclease analysis and DNA hybridization using labeled B. burgdorferi whole-cell DNA (strain ATCC 35210). Several different restriction and homology patterns were observed among these isolates, indicating genotypic heterogeneity within this genus and species. It was concluded from this study that restriction endonuclease analysis of B. burgdorferi whole-cell DNA may be a reliable and accurate method for identifying strains or genotypes of the Lyme disease agent.

Citing Articles

Human and Veterinary Vaccines for Lyme Disease.

OBier N, Hatke A, Camire A, Marconi R Curr Issues Mol Biol. 2020; 42:191-222.

PMID: 33289681 PMC: 7946718. DOI: 10.21775/cimb.042.191.

Comparative genome hybridization reveals substantial variation among clinical isolates of Borrelia burgdorferi sensu stricto with different pathogenic properties.

Terekhova D, Iyer R, Wormser G, Schwartz I J Bacteriol. 2006; 188(17):6124-34.

PMID: 16923879 PMC: 1595389. DOI: 10.1128/JB.00459-06.

Isolation and characterization of Borrelia burgdorferi sensu lato strains in an area of Italy where Lyme borreliosis is endemic.

Ciceroni L, Ciarrochi S, Ciervo A, Mondarini V, Guzzo F, Caruso G J Clin Microbiol. 2001; 39(6):2254-60.

PMID: 11376066 PMC: 88120. DOI: 10.1128/JCM.39.6.2254-2260.2001.

Genetic characteristics of Borrelia coriaceae isolates from the soft tick Ornithodoros coriaceus (Acari: Argasidae).

Hendson M, Lane R J Clin Microbiol. 2000; 38(7):2678-82.

PMID: 10878063 PMC: 86996. DOI: 10.1128/JCM.38.7.2678-2682.2000.

Antigenic and genomic analysis of a Borrelia burgdorferi sensu stricto strain isolated from Ixodes ricinus ticks in Alto Adige-South Tyrol, Italy.

Ciceroni L, Ciarrocchi S, Simeoni J Eur J Epidemiol. 1998; 14(5):511-7.

PMID: 9744686 DOI: 10.1023/a:1007432408746.

References

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

Schwan T, BURGDORFER W, Garon C . Changes in infectivity and plasmid profile of the Lyme disease spirochete, Borrelia burgdorferi, as a result of in vitro cultivation. Infect Immun. 1988; 56(8):1831-6. PMC: 259490. DOI: 10.1128/iai.56.8.1831-1836.1988. View

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

BURGDORFER W, Barbour A, Hayes S, Benach J, GRUNWALDT E, Davis J . Lyme disease-a tick-borne spirochetosis?. Science. 1982; 216(4552):1317-9. DOI: 10.1126/science.7043737. View

Ackermann R, Kabatzki J, Boisten H, Steere A, Grodzicki R, Hartung S . [Spirochete etiology of erythema chronicum migrans disease]. Dtsch Med Wochenschr. 1984; 109(3):92-7. DOI: 10.1055/s-2008-1069145. View

Schmid G, Steigerwalt A, Johnson S, Barbour A, Steere A, ROBINSON I . DNA characterization of the spirochete that causes Lyme disease. J Clin Microbiol. 1984; 20(2):155-8. PMC: 271275. DOI: 10.1128/jcm.20.2.155-158.1984. View

Steere A, Malawista S, Bartenhagen N, Spieler P, Newman J, Rahn D . The clinical spectrum and treatment of Lyme disease. Yale J Biol Med. 1984; 57(4):453-61. PMC: 2590003. View

Weber K, SCHIERZ G, Wilske B, Preac-Mursic V . European erythema migrans disease and related disorders. Yale J Biol Med. 1984; 57(4):463-71. PMC: 2590035. View

Barbour A . Isolation and cultivation of Lyme disease spirochetes. Yale J Biol Med. 1984; 57(4):521-5. PMC: 2589996. View

10.

Thiermann A, Handsaker A, Moseley S, Kingscote B . New method for classification of leptospiral isolates belonging to serogroup pomona by restriction endonuclease analysis: serovar kennewicki. J Clin Microbiol. 1985; 21(4):585-7. PMC: 271725. DOI: 10.1128/jcm.21.4.585-587.1985. View

11.

Barbour A, Heiland R, Howe T . Heterogeneity of major proteins in Lyme disease borreliae: a molecular analysis of North American and European isolates. J Infect Dis. 1985; 152(3):478-84. DOI: 10.1093/infdis/152.3.478. View

12.

Le Febvre R, FOLEY J, Thiermann A . Rapid and simplified protocol for isolation and characterization of leptospiral chromosomal DNA for taxonomy and diagnosis. J Clin Microbiol. 1985; 22(4):606-8. PMC: 268476. DOI: 10.1128/jcm.22.4.606-608.1985. View

13.

Thiermann A, Handsaker A, FOLEY J, White F, Kingscote B . Reclassification of North American leptospiral isolates belonging to serogroups Mini and Sejroe by restriction endonuclease analysis. Am J Vet Res. 1986; 47(1):61-6. View

14.

Vieyres C, Allal J, Coisne D, Thomas P, Neau J, Breux J . [European aspects of Lyme disease. 8 cases]. Presse Med. 1987; 16(2):59-62. View

15.

Wilske B, Preac-Mursic V, SCHIERZ G, Busch K . Immunochemical and immunological analysis of European Borrelia burgdorferi strains. Zentralbl Bakteriol Mikrobiol Hyg A. 1986; 263(1-2):92-102. DOI: 10.1016/s0176-6724(86)80108-0. View

16.

Anderson J, Johnson R, Magnarelli L, Hyde F, Andreadis T . New infectious spirochete isolated from short-tailed shrews and white-footed mice. J Clin Microbiol. 1987; 25(8):1490-4. PMC: 269255. DOI: 10.1128/jcm.25.8.1490-1494.1987. View

17.

Schwan T, BURGDORFER W . Antigenic changes of Borrelia burgdorferi as a result of in vitro cultivation. J Infect Dis. 1987; 156(5):852-3. DOI: 10.1093/infdis/156.5.852-a. View

18.

Bissett M, Hill W . Characterization of Borrelia burgdorferi strains isolated from Ixodes pacificus ticks in California. J Clin Microbiol. 1987; 25(12):2296-301. PMC: 269474. DOI: 10.1128/jcm.25.12.2296-2301.1987. View

19.

Barbour A . Plasmid analysis of Borrelia burgdorferi, the Lyme disease agent. J Clin Microbiol. 1988; 26(3):475-8. PMC: 266316. DOI: 10.1128/jcm.26.3.475-478.1988. View

20.

Wilske B, Preac-Mursic V, SCHIERZ G, Gueye W, Herzer P, Weber K . [Immunochemical analysis of the immune response in late manifestations of Lyme borreliosis]. Zentralbl Bakteriol Mikrobiol Hyg A. 1988; 267(4):549-58. View