MiR-155 Promotes the Growth of Osteosarcoma in a HBP1-dependent Mechanism

Overview

Journal Mol Cell Biochem

Publisher Springer

Specialty Biochemistry

Date 2015 Feb 11

PMID 25666090

Citations 13

Authors

Xiaohui Sun

Xiaolin Geng

Jun Zhang

Hongxing Zhao

Ying Liu

Affiliations

Soon will be listed here.

Abstract

Osteosarcoma (OS) is a type of malignant tumor arising from soft-tissues of bone and displays poor prognosis in most cases. However, the molecular mechanism by which OS initiates and progresses is still not completely elucidated. miR-155 has been shown to be overexpressed in OS specimen and cell lines. Our study is intended to explore the role of miR-155 in OS etiology. The data confirmed that miR-155 abundance is higher in OS samples than non-cancerous bone tissue. Inhibition of miR-155 suppressed the proliferation of OS cells and cell cycle progression in vitro, and the growth of OS xenografts in vivo. Wnt pathway was suppressed in OS cells by miR-155 inhibitors. HMG-box transcription factor 1 (HBP1), a strong Wnt pathway suppressor, was found to be a target of miR-155. Restoration of HBP1 abolished the effect of miR-155 on OS cells. Finally, miR-155 levels in OS tissues and serum are both inversely associated with the survival of OS patients. Collectively, miR-155 was identified to be among the list of OS-related oncogenic miRNAs, and HBP1-mediated Wnt signaling is involved with the role of miR-155 in OS progression.

Citing Articles

MicroRNA-155 Inhibition Activates Wnt/β-Catenin Signaling to Restore Th17/Treg Cell Balance and Protect against Acute Ischemic Stroke.

Huang W, Hong Q, Wang H, Zhu Z, Gong S eNeuro. 2025; 12(2).

PMID: 39788733 PMC: 11842039. DOI: 10.1523/ENEURO.0347-24.2024.

The role of miR-155 in cardiovascular diseases: Potential diagnostic and therapeutic targets.

Zhang R, Wang W, Li J, Li R, Zhang J, Wu Y Int J Cardiol Cardiovasc Risk Prev. 2025; 24:200355.

PMID: 39760132 PMC: 11699627. DOI: 10.1016/j.ijcrp.2024.200355.

Research Progress of Exosome-Loaded miRNA in Osteosarcoma.

Yao P, Lu Y, Cai Z, Yu T, Kang Y, Zhang Y Cancer Control. 2022; 29:10732748221076683.

PMID: 35179996 PMC: 8859673. DOI: 10.1177/10732748221076683.

miR-29c&b2 encourage extramedullary infiltration resulting in the poor prognosis of acute myeloid leukemia.

Wei Y, Lu W, Yu Y, Zhai Y, Guo H, Yang S Oncogene. 2021; 40(19):3434-3448.

PMID: 33888868 DOI: 10.1038/s41388-021-01775-9.

MiR-155 promotes colitis-associated intestinal fibrosis by targeting HBP1/Wnt/β-catenin signalling pathway.

Li N, Ouyang Y, Xu X, Yuan Z, Liu C, Zhu Z J Cell Mol Med. 2021; 25(10):4765-4775.

PMID: 33769664 PMC: 8107084. DOI: 10.1111/jcmm.16445.

References

Yan Z, Wang J, Wang C, Jiao Y, Qi W, Che S . miR-96/HBP1/Wnt/β-catenin regulatory circuitry promotes glioma growth. FEBS Lett. 2014; 588(17):3038-46. DOI: 10.1016/j.febslet.2014.06.017. View

Ma L, Liu J, Shen J, Liu L, Wu J, Li W . Expression of miR-122 mediated by adenoviral vector induces apoptosis and cell cycle arrest of cancer cells. Cancer Biol Ther. 2010; 9(7):554-61. DOI: 10.4161/cbt.9.7.11267. View

Gironella M, Seux M, Xie M, Cano C, Tomasini R, Gommeaux J . Tumor protein 53-induced nuclear protein 1 expression is repressed by miR-155, and its restoration inhibits pancreatic tumor development. Proc Natl Acad Sci U S A. 2007; 104(41):16170-5. PMC: 2042180. DOI: 10.1073/pnas.0703942104. View

Tian J, He H, Lei G . Wnt/β-catenin pathway in bone cancers. Tumour Biol. 2014; 35(10):9439-45. DOI: 10.1007/s13277-014-2433-8. View

Liu J, Ma L, Li C, Zhang Z, Yang G, Zhang W . Tumor-targeting TRAIL expression mediated by miRNA response elements suppressed growth of uveal melanoma cells. Mol Oncol. 2013; 7(6):1043-55. PMC: 5528445. DOI: 10.1016/j.molonc.2013.08.003. View

He J, Hao Y, Wang X, Yang X, Shao J, Guo F . Review of the molecular pathogenesis of osteosarcoma. Asian Pac J Cancer Prev. 2014; 15(15):5967-76. DOI: 10.7314/apjcp.2014.15.15.5967. View

Chen L, Jiang K, Jiang H, Wei P . miR-155 mediates drug resistance in osteosarcoma cells via inducing autophagy. Exp Ther Med. 2014; 8(2):527-532. PMC: 4079430. DOI: 10.3892/etm.2014.1752. View

Ma L, Liu J, Liu L, Duan G, Wang Q, Xu Y . Overexpression of the transcription factor MEF2D in hepatocellular carcinoma sustains malignant character by suppressing G2-M transition genes. Cancer Res. 2014; 74(5):1452-62. DOI: 10.1158/0008-5472.CAN-13-2171. View

Yang Z, Wu L, Zhu X, Xu J, Jin R, Li G . MiR-29a modulates the angiogenic properties of human endothelial cells. Biochem Biophys Res Commun. 2013; 434(1):143-9. PMC: 3646542. DOI: 10.1016/j.bbrc.2013.03.054. View

10.

Wang B, Liu J, Ma L, Xiao H, Wang Y, Li Y . Chimeric 5/35 adenovirus-mediated Dickkopf-1 overexpression suppressed tumorigenicity of CD44⁺ gastric cancer cells via attenuating Wnt signaling. J Gastroenterol. 2012; 48(7):798-808. DOI: 10.1007/s00535-012-0711-z. View

11.

Lauvrak S, Munthe E, Kresse S, Stratford E, Namlos H, Meza-Zepeda L . Functional characterisation of osteosarcoma cell lines and identification of mRNAs and miRNAs associated with aggressive cancer phenotypes. Br J Cancer. 2013; 109(8):2228-36. PMC: 3798956. DOI: 10.1038/bjc.2013.549. View

12.

Faraoni I, Antonetti F, Cardone J, Bonmassar E . miR-155 gene: a typical multifunctional microRNA. Biochim Biophys Acta. 2009; 1792(6):497-505. DOI: 10.1016/j.bbadis.2009.02.013. View

13.

Wang B, Majumder S, Nuovo G, Kutay H, Volinia S, Patel T . Role of microRNA-155 at early stages of hepatocarcinogenesis induced by choline-deficient and amino acid-defined diet in C57BL/6 mice. Hepatology. 2009; 50(4):1152-61. PMC: 2757532. DOI: 10.1002/hep.23100. View

14.

Lin C, Ji T, Chen C, Hoang B . Wnt signaling in osteosarcoma. Adv Exp Med Biol. 2014; 804:33-45. DOI: 10.1007/978-3-319-04843-7_2. View

15.

Li C, Chunbao L, Cheng Q, Qianpeng C, Liu J, Jia L . Potent growth-inhibitory effect of TRAIL therapy mediated by double-regulated oncolytic adenovirus on osteosarcoma. Mol Cell Biochem. 2012; 364(1-2):337-44. DOI: 10.1007/s11010-012-1235-9. View

16.

Ebert M, Sharp P . Roles for microRNAs in conferring robustness to biological processes. Cell. 2012; 149(3):515-24. PMC: 3351105. DOI: 10.1016/j.cell.2012.04.005. View

17.

McQueen P, Ghaffar S, Guo Y, Rubin E, Zi X, Hoang B . The Wnt signaling pathway: implications for therapy in osteosarcoma. Expert Rev Anticancer Ther. 2011; 11(8):1223-32. DOI: 10.1586/era.11.94. View

18.

Vincent K, Pichler M, Lee G, Ling H . MicroRNAs, genomic instability and cancer. Int J Mol Sci. 2014; 15(8):14475-91. PMC: 4159863. DOI: 10.3390/ijms150814475. View

19.

Luetke A, Meyers P, Lewis I, Juergens H . Osteosarcoma treatment - where do we stand? A state of the art review. Cancer Treat Rev. 2013; 40(4):523-32. DOI: 10.1016/j.ctrv.2013.11.006. View

20.

Yang M, Shen H, Qiu C, Ni Y, Wang L, Dong W . High expression of miR-21 and miR-155 predicts recurrence and unfavourable survival in non-small cell lung cancer. Eur J Cancer. 2012; 49(3):604-15. DOI: 10.1016/j.ejca.2012.09.031. View