Diversities of Interaction of Murine Macrophages with Three Strains of Candida Albicans Represented by MyD88, CARD9 Gene Expressions and ROS, IL-10 and TNF-α Secretion

Overview

Journal Int J Clin Exp Med

Specialty General Medicine

Date 2015 Feb 10

PMID 25664026

Citations 2

Authors

Xiaohuan Zhang

Yanping Ge

Wenqing Li

Yan Hu

Affiliations

Soon will be listed here.

Abstract

Aim: To explore the mechanisms underlying the different responses of macrophages to distinct Candida albicans strains.

Methods: Bone marrow was collected from mice. Macrophages were independently incubated with 3 Candida albicans strains.

Results: MyD88 expression in Candida albicans 3683 group was significantly higher than that in Candida albicans 3630 group and Candida albicans SC5314 group, and marked difference was also observed between later two groups (P<0.05). CARD9 expression in Candida albicans 3630 group was higher than that in Candida albicans 3683 group and Candida albicans SC5314 group. Fluorescence intensity was 46.78±0.79 in Candida albicans 3630 group, 32.60±1.31 in Candida albicans 3683 group and 19.40±0.58 in Candida albicans SC5314, and significant difference was observed between any two groups (P<0.05). TNF-α and IL-10 were 18.9843±0.7081 pg/ml and 11.6690±0.3167 pg/ml, respectively, in Candida albicans 3683 group, which were markedly higher than those in Candida albicans 3630 group and Candida albicans SC5314 group (P<0.05 and 0.01).

Conclusion: Different Candida albicans strains may induce CARD9 expression and alter the production of ROS, TNF-α and IL-10 in macrophages, which may be one of mechanisms underlying the different killing effects of macrophages on distinct Candida albicans strains.

Citing Articles

CARD9 Signaling, Inflammation, and Diseases.

Liu X, Jiang B, Hao H, Liu Z Front Immunol. 2022; 13:880879.

PMID: 35432375 PMC: 9005907. DOI: 10.3389/fimmu.2022.880879.

[Progress on the role of Toll-like receptors in Candida albicans infections].

Yun Z, Jianping P Zhejiang Da Xue Xue Bao Yi Xue Ban. 2016; 45(3):302-7.

PMID: 27651197 PMC: 10396928. DOI: 10.3785/j.issn.1008-9292.2016.05.14.

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