Contribution of Cytochromes and Proteins to the Effect of Ascorbic Acid on Artificial and Microsomal Hydroxylation Systems Containing Oxygen and Hydrogen Peroxide

Overview

Journal Biochem J

Specialty Biochemistry

Date 1978 Mar 15

PMID 25655

Authors

J Chrastil

J T Wilson

Affiliations

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Abstract

Hydroxylation systems containing cytochromes, proteins and ascorbic acid were studied at physiological pH (7.4) under O2 or N2 with added H2O2. Proteins inhibited aromatic hydroxylation of p-nitrophenol or oxidative demethylation of ethylmorphine in ascorbic acid-containing systems incubated under O2, but strongly activated the systems containing H2O2. Cytochrome c and partially purified cytochrome P-450 from rat liver microsomal preparations activated the system in either O2 or H2O2. The systems needed ascorbic acid (or other enol structures) for activation. Cytochrome iron participated probably in the activation of O2, whereas cytochrome protein participated in a free radical activation of H2O2 (or of O2).

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