Nd(III)-induced Rice Mitochondrial Dysfunction Investigated by Spectroscopic and Microscopic Methods

Overview

Journal J Membr Biol

Date 2015 Feb 5

PMID 25650179

Citations 3

Authors

Cai-Fen Xia

Long Lv

Xin-You Chen

Bo-Qiao Fu

Ke-Lin Lei

Cai-Qin Qin

Yi Liu

Affiliations

Soon will be listed here.

Abstract

The production capacity and yield of neodymium (Nd) in China have ranked the first in the world. Because of its unique biophysical and biochemical properties, Nd compounds have entered into the agricultural environment greatly to promote plant growth. Mitochondria play a crucial role in respiration and metabolism during the growth of plants. However, little is known about the mechanism by which Nd act at the mitochondrial level in plant cells. In this study, rice mitochondrial swelling, collapsed transmembrane potential and decreased membrane fluidity were examined to be important factors for mitochondria permeability transition pore (mPTP) opening induced by Nd(III). The protection of cyclosporin A (CsA) and dithiothreitol (DTT) could confirm that Nd(III) could trigger mPTP opening. Additionally, mitochondrial membrane breakdown observed by TEM and the release of cytochrome c (Cyt c) could also elucidate the mPTP opening from another point of view. At last, the study showed that Nd(III) could restrain the mitochondrial membrane lipid peroxide, so it might interact with anionic lipid too. This detection will be conductive to the safe application of Nd compounds in agriculture and food industry.

Citing Articles

Rare earth metallic elements in plants: assessing benefits, risks and mitigating strategies.

Kaur P, Mahajan M, Gambhir H, Khan A, Khan M Plant Cell Rep. 2024; 43(9):216.

PMID: 39145796 DOI: 10.1007/s00299-024-03305-9.

Antimicrobial peptide CGA-N12 decreases the Candida tropicalis mitochondrial membrane potential via mitochondrial permeability transition pore.

Li R, Zhao J, Huang L, Yi Y, Li A, Li D Biosci Rep. 2020; 40(5).

PMID: 32368781 PMC: 7225414. DOI: 10.1042/BSR20201007.

Study of mitochondrial swelling, membrane fluidity and ROS production induced by nano-TiO and prevented by Fe incorporation.

Barkhade T, Mahapatra S, Banerjee I Toxicol Res (Camb). 2019; 8(5):711-722.

PMID: 31588348 PMC: 6764469. DOI: 10.1039/c9tx00143c.

References

Zhu Y, Xu H, Huang K . Mitochondrial permeability transition and cytochrome c release induced by selenite. J Inorg Biochem. 2002; 90(1-2):43-50. DOI: 10.1016/s0162-0134(02)00407-5. View

Bernardi P . The permeability transition pore. Control points of a cyclosporin A-sensitive mitochondrial channel involved in cell death. Biochim Biophys Acta. 1996; 1275(1-2):5-9. DOI: 10.1016/0005-2728(96)00041-2. View

Vianello A, Macri F, Braidot E, Mokhova E . Effect of 6-ketocholestanol on FCCP- and DNP-induced uncoupling in plant mitochondria. FEBS Lett. 1995; 365(1):7-9. DOI: 10.1016/0014-5793(95)00431-8. View

Ma Z, Guan Y, Liu X, Liu H . Synthesis of magnetic chelator for high-capacity immobilized metal affinity adsorption of protein by cerium initiated graft polymerization. Langmuir. 2005; 21(15):6987-94. DOI: 10.1021/la050504g. View

Liu X, Li J, Zhang Y, Ge Y, Tian F, Dai J . Mitochondrial permeability transition induced by different concentrations of zinc. J Membr Biol. 2011; 244(3):105-12. DOI: 10.1007/s00232-011-9403-5. View

Ricchelli F, Gobbo S, Moreno G, Salet C . Changes of the fluidity of mitochondrial membranes induced by the permeability transition. Biochemistry. 1999; 38(29):9295-300. DOI: 10.1021/bi9900828. View

Kale S, Arora S, Bhayani K, Paknikar K, Jani M, Wagh U . Cerium doping and stoichiometry control for biomedical use of La0.7Sr0.3MnO3 nanoparticles: microwave absorption and cytotoxicity study. Nanomedicine. 2007; 2(4):217-21. DOI: 10.1016/j.nano.2006.10.001. View

Taresa E, Kedei N, Thomazy V, Fesus L . An involucrin-like protein in hepatocytes serves as a substrate for tissue transglutaminase during apoptosis. J Biol Chem. 1992; 267(36):25648-51. View

Gerencser A, Doczi J, Torocsik B, Bossy-Wetzel E, Adam-Vizi V . Mitochondrial swelling measurement in situ by optimized spatial filtering: astrocyte-neuron differences. Biophys J. 2008; 95(5):2583-98. PMC: 2517017. DOI: 10.1529/biophysj.107.118620. View

10.

Kulikov A, Shilov E, Mufazalov I, Gogvadze V, Nedospasov S, Zhivotovsky B . Cytochrome c: the Achilles' heel in apoptosis. Cell Mol Life Sci. 2011; 69(11):1787-97. PMC: 11114681. DOI: 10.1007/s00018-011-0895-z. View

11.

Jutila A, Rytomaa M, Kinnunen P . Detachment of cytochrome c by cationic drugs from membranes containing acidic phospholipids: comparison of lidocaine, propranolol, and gentamycin. Mol Pharmacol. 1998; 54(4):722-32. View

12.

Zoratti M, Szabo I, De Marchi U . Mitochondrial permeability transitions: how many doors to the house?. Biochim Biophys Acta. 2004; 1706(1-2):40-52. DOI: 10.1016/j.bbabio.2004.10.006. View

13.

Niki E, Yoshida Y, Saito Y, Noguchi N . Lipid peroxidation: mechanisms, inhibition, and biological effects. Biochem Biophys Res Commun. 2005; 338(1):668-76. DOI: 10.1016/j.bbrc.2005.08.072. View

14.

Zoratti M, Szabo I . The mitochondrial permeability transition. Biochim Biophys Acta. 1995; 1241(2):139-76. DOI: 10.1016/0304-4157(95)00003-a. View

15.

Arpagaus S, Rawyler A, Braendle R . Occurrence and characteristics of the mitochondrial permeability transition in plants. J Biol Chem. 2001; 277(3):1780-7. DOI: 10.1074/jbc.M109416200. View

16.

Yuan H, Williams S, Adachi S, Oltersdorf T, Gottlieb R . Cytochrome c dissociation and release from mitochondria by truncated Bid and ceramide. Mitochondrion. 2005; 2(4):237-44. DOI: 10.1016/S1567-7249(02)00106-X. View

17.

Pastore D, Stoppelli M, Di Fonzo N, Passarella S . The existence of the K(+) channel in plant mitochondria. J Biol Chem. 1999; 274(38):26683-90. DOI: 10.1074/jbc.274.38.26683. View

18.

Malekova L, Kominkova V, Ferko M, Stefanik P, Krizanova O, Ziegelhoffer A . Bongkrekic acid and atractyloside inhibits chloride channels from mitochondrial membranes of rat heart. Biochim Biophys Acta. 2006; 1767(1):31-44. DOI: 10.1016/j.bbabio.2006.10.004. View

19.

Leung A, Halestrap A . Recent progress in elucidating the molecular mechanism of the mitochondrial permeability transition pore. Biochim Biophys Acta. 2008; 1777(7-8):946-52. DOI: 10.1016/j.bbabio.2008.03.009. View

20.

Passarella S, Atlante A, Valenti D, de Bari L . The role of mitochondrial transport in energy metabolism. Mitochondrion. 2005; 2(5):319-43. DOI: 10.1016/S1567-7249(03)00008-4. View