» Articles » PMID: 25647097

Abscission is Regulated by the ESCRT-III Protein Shrub in Drosophila Germline Stem Cells

Overview
Journal PLoS Genet
Specialty Genetics
Date 2015 Feb 4
PMID 25647097
Citations 33
Authors
Affiliations
Soon will be listed here.
Abstract

Abscission is the final event of cytokinesis that leads to the physical separation of the two daughter cells. Recent technical advances have allowed a better understanding of the cellular and molecular events leading to abscission in isolated yeast or mammalian cells. However, how abscission is regulated in different cell types or in a developing organism remains poorly understood. Here, we characterized the function of the ESCRT-III protein Shrub during cytokinesis in germ cells undergoing a series of complete and incomplete divisions. We found that Shrub is required for complete abscission, and that levels of Shrub are critical for proper timing of abscission. Loss or gain of Shrub delays abscission in germline stem cells (GSCs), and leads to the formation of stem-cysts, where daughter cells share the same cytoplasm as the mother stem cell and cannot differentiate. In addition, our results indicate a negative regulation of Shrub by the Aurora B kinase during GSC abscission. Finally, we found that Lethal giant discs (lgd), known to be required for Shrub function in the endosomal pathway, also regulates the duration of abscission in GSCs.

Citing Articles

Increased levels of lagging strand polymerase α in an adult stem cell lineage affect replication-coupled histone incorporation.

Davis B, Snedeker J, Ranjan R, Wooten M, Barton S, Blundon J Sci Adv. 2025; 11(9):eadu6799.

PMID: 40020063 PMC: 11870066. DOI: 10.1126/sciadv.adu6799.


Lineage-based scaling of germline intercellular bridges during oogenesis.

Shaikh U, Sherlock K, Wilson J, Gilliland W, Lewellyn L Development. 2024; 151(16).

PMID: 39190553 PMC: 11385318. DOI: 10.1242/dev.202676.


Structural Analysis of the ESCRT-III Regulator Lethal(2) Giant Discs/Coiled-Coil and C2 Domain-Containing Protein 1 (Lgd/CC2D1).

Breuer T, Tibbe C, Troost T, Klein T Cells. 2024; 13(14.

PMID: 39056756 PMC: 11275157. DOI: 10.3390/cells13141174.


Reduced Levels of Lagging Strand Polymerases Shape Stem Cell Chromatin.

Snedeker J, Davis B, Ranjan R, Wooten M, Blundon J, Chen X bioRxiv. 2024; .

PMID: 38746451 PMC: 11092439. DOI: 10.1101/2024.04.26.591383.


Two distinct waves of transcriptome and translatome changes drive Drosophila germline stem cell differentiation.

Samuels T, Gui J, Gebert D, Karam Teixeira F EMBO J. 2024; 43(8):1591-1617.

PMID: 38480936 PMC: 11021484. DOI: 10.1038/s44318-024-00070-z.


References
1.
Huynh J, Shulman J, Benton R, St Johnston D . PAR-1 is required for the maintenance of oocyte fate in Drosophila. Development. 2001; 128(7):1201-9. DOI: 10.1242/dev.128.7.1201. View

2.
McKearin D, Ohlstein B . A role for the Drosophila bag-of-marbles protein in the differentiation of cystoblasts from germline stem cells. Development. 1995; 121(9):2937-47. DOI: 10.1242/dev.121.9.2937. View

3.
Chen D, McKearin D . A discrete transcriptional silencer in the bam gene determines asymmetric division of the Drosophila germline stem cell. Development. 2003; 130(6):1159-70. DOI: 10.1242/dev.00325. View

4.
Morita E, Sandrin V, Chung H, Morham S, Gygi S, Rodesch C . Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis. EMBO J. 2007; 26(19):4215-27. PMC: 2230844. DOI: 10.1038/sj.emboj.7601850. View

5.
Murray M, Saint R . Photoactivatable GFP resolves Drosophila mesoderm migration behaviour. Development. 2007; 134(22):3975-83. DOI: 10.1242/dev.005389. View