The GroES and GroEL Heat Shock Gene Products of Escherichia Coli Are Essential for Bacterial Growth at All Temperatures

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1989 Mar 1

PMID 2563997

Citations 238

Authors

O Fayet

T Ziegelhoffer

C Georgopoulos

Affiliations

Soon will be listed here.

Abstract

The products of the groES and groEL genes of Escherichia coli, constituting the groE operon, are known to be required for growth at high temperature (42 degrees C) and are members of the heat shock regulon. Using a genetic approach, we examined the requirement for these gene products for bacterial growth at low temperature (17 to 30 degrees C). To do this, we constructed various groES groEL heterodiploid derivative strains. By inactivating one of the groE operons by a polar insertion, it was shown by bacteriophage P1 transduction that at least one of the groE genes was essential for growth at low temperature. Further P1 transduction experiments with strains that were heterodiploid for only one of the groE genes demonstrated that both groE gene products were required for growth at low temperature, which suggested a fundamental role for the groE proteins in E. coli growth and physiology.

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References

Takano T, KAKEFUDA T . Involvement of a bacterial factor in morphogenesis of bacteriophage capsid. Nat New Biol. 1972; 239(89):34-7. DOI: 10.1038/newbio239034a0. View

Kusukawa N, Yura T . Heat shock protein GroE of Escherichia coli: key protective roles against thermal stress. Genes Dev. 1988; 2(7):874-82. DOI: 10.1101/gad.2.7.874. View

Georgopoulos C, Hendrix R, Casjens S, Kaiser A . Host participation in bacteriophage lambda head assembly. J Mol Biol. 1973; 76(1):45-60. DOI: 10.1016/0022-2836(73)90080-6. View

ZWEIG M, Cummings D . Cleavage of head and tail proteins during bacteriophage T5 assembly: selective host involvement in the cleavage of a tail protein. J Mol Biol. 1973; 80(3):505-18. DOI: 10.1016/0022-2836(73)90418-x. View

Georgopoulos C, Eisen H . Bacterial mutants which block phage assembly. J Supramol Struct. 1974; 2(2-4):349-59. DOI: 10.1002/jss.400020224. View

Revel H, Stitt B, LIELAUSIS I, Wood W . Role of the host cell in bacteriophage T4 development. I. Characterization of host mutants that block T4 head assembly. J Virol. 1980; 33(1):366-76. PMC: 288553. DOI: 10.1128/JVI.33.1.366-376.1980. View

Tilly K, Murialdo H, Georgopoulos C . Identification of a second Escherichia coli groE gene whose product is necessary for bacteriophage morphogenesis. Proc Natl Acad Sci U S A. 1981; 78(3):1629-33. PMC: 319185. DOI: 10.1073/pnas.78.3.1629. View

Burnette W . "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981; 112(2):195-203. DOI: 10.1016/0003-2697(81)90281-5. View

Ish-Horowicz D, Burke J . Rapid and efficient cosmid cloning. Nucleic Acids Res. 1981; 9(13):2989-98. PMC: 327326. DOI: 10.1093/nar/9.13.2989. View

10.

Hawkes R, Niday E, Gordon J . A dot-immunobinding assay for monoclonal and other antibodies. Anal Biochem. 1982; 119(1):142-7. DOI: 10.1016/0003-2697(82)90677-7. View

11.

VanBogelen R, Vaughn V, Neidhardt F . Gene for heat-inducible lysyl-tRNA synthetase (lysU) maps near cadA in Escherichia coli. J Bacteriol. 1983; 153(2):1066-8. PMC: 221734. DOI: 10.1128/jb.153.2.1066-1068.1983. View

12.

Bachmann B . Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev. 1983; 47(2):180-230. PMC: 281571. DOI: 10.1128/mr.47.2.180-230.1983. View

13.

Kochan J, Murialdo H . Early intermediates in bacteriophage lambda prohead assembly. II. Identification of biologically active intermediates. Virology. 1983; 131(1):100-15. DOI: 10.1016/0042-6822(83)90537-8. View

14.

Wada M, Itikawa H . Participation of Escherichia coli K-12 groE gene products in the synthesis of cellular DNA and RNA. J Bacteriol. 1984; 157(2):694-6. PMC: 215308. DOI: 10.1128/jb.157.2.694-696.1984. View

15.

Kochan J, Carrascosa J, Murialdo H . Bacteriophage lambda preconnectors. Purification and structure. J Mol Biol. 1984; 174(3):433-47. DOI: 10.1016/0022-2836(84)90330-9. View

16.

Joyce C, Grindley N . Method for determining whether a gene of Escherichia coli is essential: application to the polA gene. J Bacteriol. 1984; 158(2):636-43. PMC: 215477. DOI: 10.1128/jb.158.2.636-643.1984. View

17.

Kanaya S, Crouch R . The rnh gene is essential for growth of Escherichia coli. Proc Natl Acad Sci U S A. 1984; 81(11):3447-51. PMC: 345525. DOI: 10.1073/pnas.81.11.3447. View

18.

Jasin M, Schimmel P . Deletion of an essential gene in Escherichia coli by site-specific recombination with linear DNA fragments. J Bacteriol. 1984; 159(2):783-6. PMC: 215717. DOI: 10.1128/jb.159.2.783-786.1984. View

19.

Grossman A, Erickson J, Gross C . The htpR gene product of E. coli is a sigma factor for heat-shock promoters. Cell. 1984; 38(2):383-90. DOI: 10.1016/0092-8674(84)90493-8. View

20.

Russel M, Model P . Replacement of the fip gene of Escherichia coli by an inactive gene cloned on a plasmid. J Bacteriol. 1984; 159(3):1034-9. PMC: 215764. DOI: 10.1128/jb.159.3.1034-1039.1984. View